Angiotensin II inhibits the protein expression of ZO‑1 in vascular endothelial cells by downregulating VE‑cadherin

Molecular Medicine Reports
Longbin LiuHangyuan Guo

Abstract

Angiotensin II (Ang II) is reported to be involved in the development of various cardiovascular diseases by disrupting microvessel permeability, however, the underlying mechanism remains to be elucidated. The present study aimed to investigate the mechanism by which Ang II disrupts microvascular permeability. Rat endothelial cells were subjected to primary culture and identification. Cells in passages 4‑7 were then used for the following experiments. The cells were divided into control, Ang II, and Ang II + valsartan groups, and reverse transcription‑quantitative polymerase chain reaction and western blot analyses were perform to evaluate the expression of zonula occludens‑1 (ZO‑1) and vascular endothelial (VE)‑cadherin in the cells. The distribution of ZO‑1 protein was also detected using immunofluorescence assays. It was found that, compared with the control group, lower expression levels of ZO‑1 and VE‑cadherin were present in the Ang II group (P<0.01). ZO‑1 was also irregularly distributed at the periphery of the cells. In addition, the overexpression of VE‑cadherin reversed the effect of Ang II on the expression and distribution of ZO‑1 in endothelial cells. Together, these results suggested that Ang II inhibited the prote...Continue Reading

References

Jan 1, 1994·Breast Cancer Research and Treatment·C L SommersE P Gelmann
Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
Jun 22, 2005·Thrombosis and Haemostasis·Andrzej MogielnickiWlodzimierz Buczko
Aug 9, 2006·The Journal of Cell Biology·Maria Grazia LampugnaniElisabetta Dejana
Jun 10, 2009·Circulation·Alun JonesGillian W Cockerill
Nov 23, 2011·Proceedings of the National Academy of Sciences of the United States of America·Linlin SuC Yan Cheng
Jul 4, 2015·Circulation Research·Philipp Du BoisJens Fielitz
Nov 22, 2015·Indian Journal of Pediatrics·Zhijiang QiXiaozhi Wang
Jul 29, 2016·Molecular Biology of the Cell·Benjamin F BrinkmannKlaus Ebnet
Sep 13, 2016·Medical Science Monitor : International Medical Journal of Experimental and Clinical Research·Jian DuJunjie Qin
Mar 8, 2017·Development·Loïc SauteurHeinz-Georg Belting
Mar 17, 2017·Experimental Cell Research·Andrew Vae PriestSanjeevi Sivasankar
Mar 23, 2017·Molecular Cancer·Daniel Delgado-BellidoF Javier Oliver

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