Angiotensin II regulates tyrosine hydroxylase activity and mRNA expression in rat mediobasal hypothalamic cultures: the role of specific protein kinases

Journal of Neurochemistry
Frank Y MaStephen J Bunn

Abstract

Dopamine secreted by hypothalamic neurons is crucial in regulating prolactin secretion from the pituitary. We have examined the ability of angiotensin II (AngII) to regulate the activity of these dopaminergic neurons and thus act as a potential physiological regulator of prolactin secretion. Using a hypothalamic cell culture preparation we determined the effect of AngII on tyrosine hydroxylase activity and expression (TOH). This is important because TOH is the rate-limiting enzyme in dopamine biosynthesis. AngII stimulated a time- and concentration-dependent increase in TOH activity which was suppressed by inhibitors able to act on protein kinase A (PKA), protein kinase C (PKC) and Ca(2+)/calmodulin-dependent protein kinase II (CaMPKII). An inhibitor of the mitogen-activated protein kinase (MAPK) pathway, PD 98059, reduced basal TOH activity but the AngII response was still detectable. AngII stimulation enhanced the phosphorylation of TOH at Ser19, Ser31 and Ser40. AngII also induced a time-dependent increase in TOH mRNA expression which was unaffected by inhibitors able to act on PKA and CaMPKII, but was abolished by inhibitors able to act on ERK and PKC. AngII responses were very much larger in cultures prepared from female w...Continue Reading

Citations

Mar 15, 2008·The Journal of Biological Chemistry·Karen L MurphyMarc G Caron
Jul 5, 2008·American Journal of Physiology. Heart and Circulatory Physiology·Baojian XueMeredith Hay
Nov 29, 2011·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·Zhang SuojunLei Ting
Dec 1, 2004·Journal of Neurochemistry·Peter R DunkleyPhillip W Dickson
Feb 3, 2009·Journal of Neurochemistry·María A Lopez VerrilliMariela M Gironacci
Oct 16, 2004·Biochemical and Biophysical Research Communications·Gonzalo HerradonThomas F Deuel
Sep 29, 2007·Biochemical and Biophysical Research Communications·Tom N GrammatopoulosDavid G Standaert
Nov 18, 2015·Reviews in the Neurosciences·Anjana Bali, Amteshwar Singh Jaggi

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