Angiotensin II-stimulated collagen synthesis in aortic adventitial fibroblasts is mediated by connective tissue growth factor

Hypertension Research : Official Journal of the Japanese Society of Hypertension
Zai-qian CheDing-Liang Zhu

Abstract

Angiotensin II (Ang II), a potent mediator of vascular remodeling, can stimulate the synthesis of extracellular matrix in vascular cells. Recent studies indicate that connective tissue growth factor (CTGF) is involved in collagen synthesis. There is also increasing evidence that adventitial fibroblasts (AFs) are actively involved in vascular remodeling. However, whether collagen synthesis by AFs is mediated by CTGF, or whether it is relevant to Ang II, has not been studied. The present study was conducted to determine whether CTGF is expressed in AFs, and if so, whether the CTGF produced by AFs participates in collagen synthesis. The AFs were isolated from thoracic aorta of Wistar-Kyoto rats (WKY). The expression of CTGF was measured by Western blot or real-time PCR. Collagen synthesis was assessed by [(3)H]proline incorporation. Our results suggested that CTGF was expressed in AFs and secreted into medium. Ang II increased CTGF mRNA and protein expression in a time- and dose-dependent manner, with the maximal protein increase occurring at 24 h with an Ang II dose of 10(-7) mol/L, and this increase was inhibited by the Ang II receptor type 1 (AT(1)-R) antagonist losartan, but not by the Ang II receptor type 2 (AT(2)-R) antagoni...Continue Reading

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Citations

Dec 22, 2010·International Heart Journal·Xiao-Yan ZhaoRui-Yun Liu
Mar 26, 2016·Biochemical and Biophysical Research Communications·Rui-Qing HePing-Jin Gao
Sep 12, 2012·The International Journal of Biochemistry & Cell Biology·María Gabriela MoralesClaudio Cabello-Verrugio
Sep 19, 2014·Journal of Pharmacological Sciences·Yueqiang LiuSuzhen Zhao
Mar 28, 2009·Hypertension Research : Official Journal of the Japanese Society of Hypertension·Julio Gallego-DelgadoJesus Egido
Dec 31, 2009·Current Opinion in Nephrology and Hypertension·Ana M BrionesMercedes Salaices
Oct 15, 2010·Hypertension Research : Official Journal of the Japanese Society of Hypertension·Mutsumi IwamotoYoshifumi Ninomiya
Jun 4, 2015·The Journal of Pharmacology and Experimental Therapeutics·Goran MarinkovićVivian de Waard
Nov 17, 2020·Journal of the American Heart Association·Laura E BruijnJan H N Lindeman
Jan 31, 2021·Genes·Tyler J CreamerElena Gallo MacFarlane
Mar 2, 2021·Proceedings. Mathematical, Physical, and Engineering Sciences·Marcos LatorreJay D Humphrey

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