Angiotensin II stimulates proliferation of primary human keratinocytes via a non-AT1, non-AT2 angiotensin receptor

Biochemical and Biophysical Research Communications
U M SteckelingsB M Henz

Abstract

Angiotensin II is a hormone with long known cardiovascular actions. Recent studies revealed an additional role for angiotensin II in the regulation of cell proliferation. This study was performed to clarify whether skin is a target organ for these novel angiotensin actions. Radioligand binding studies identified a high-affinity angiotensin receptor on human primary keratinocytes in vitro with a Kd of 4.5 nM and a Bmax of 0.12 nM. Competition experiments with losartan and PD 123177 revealed that this receptor was not of the AT1- nor the AT2-subtype. Stimulation of human keratinocytes with angiotensin II (10(-10) to 10(-5) M) led to a dose dependent increase in 3H-thymidine incorporation, indicating that the keratinocyte receptor mediates a mitogenic effect. This effect is comparable at 10(-9) M to stimulation of keratinocytes by EGF (50ng/ml) and FGF (50ng/ml). These results demonstrate for the first time a possible physiological role for angiotensin II in human skin involving the regulation of keratinocyte proliferation.

Citations

Jun 26, 2001·The British Journal of Dermatology·H Takeda, S Kondo
Sep 5, 2002·The British Journal of Dermatology·C Marquart-ElbazD Lipsker
Jul 27, 2001·Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society·K RodgersG Dizerega
Dec 2, 2015·Experimental and Therapeutic Medicine·Li-Hong LiuRong-Ya Yang
Mar 12, 2015·Molecular Medicine Reports·Li-Hong LiuRong-Ya Yang
Oct 18, 2005·The British Journal of Dermatology·U M SteckelingsM Artuc
Jan 13, 2004·Experimental Dermatology·Kathleen E RodgersGere diZerega
May 29, 2004·American Journal of Obstetrics and Gynecology·Fumitaka KikkawaShigehiko Mizutani
Mar 14, 2003·Der Hautarzt; Zeitschrift für Dermatologie, Venerologie, und verwandte Gebiete·H M Ockenfels
Aug 4, 2018·Expert Opinion on Drug Safety·Brahim AzzouzThierry Trenque
Mar 25, 2000·Biulleten' eksperimental'noĭ biologii i meditsiny·S S Timoshina, E Iu Zhivotova
Jul 4, 2006·Physiological Reviews·Martin PaulReinhold Kreutz
Oct 20, 2018·Current Drug Safety·Ons CharfiAhmed Zaïem
Dec 23, 2009·American Journal of Therapeutics·Gurpreet LambaLeanne Forman
Feb 24, 1999·Journal of Cardiovascular Pharmacology·U Stroth, T Unger
Mar 7, 2000·Journal of Hypertension·T Unger
Jul 23, 2020·Experimental Dermatology·Igor Maciel Souza SilvaUlrike Muscha Steckelings
May 19, 2020·Matrix Biology : Journal of the International Society for Matrix Biology·Mohammad AlQudahMichael P Czubryt

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Clinical and Diagnostic Laboratory Immunology
Kathleen E RodgersG Dizerega
Biochemical and Biophysical Research Communications
J A NadalA G Scicli
Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society
Kathleen E RodgersG Dizerega
© 2021 Meta ULC. All rights reserved