Angiotensin II type 2 receptor gene transfer elicits cardioprotective effects in an angiotensin II infusion rat model of hypertension

Physiological Genomics
Beverly L FalcónMohan K Raizada

Abstract

The role of the angiotensin II type 2 receptor (AT2R) in cardiovascular physiology remains elusive. We have developed an in vivo lentiviral vector-mediated gene transfer system to study the physiological functions of the AT2R. Our objectives in this study were to determine whether the AT2R influences cardiac hypertrophy and myocardial and perivascular fibrosis in a nongenetic rat model of hypertension. Lentiviral vector containing the AT2R or saline was injected intracardially in 5-day-old Sprague-Dawley rats. This resulted in a persistent overexpression of the AT2R in cardiac tissues. At 15 wk of age, animals were infused with either 200 ng x kg(-1) x min(-1) of angiotensin II or saline by implantation of a 4-wk osmotic minipump. This resulted in an increase in blood pressure (BP) that reached maximal by 2 wk of treatment and was associated with a 123% increase in left ventricular wall thickness (LVWT) and a 129% increase in heart weight to body weight ratios (HW/BW). In addition, the increase in cardiac hypertrophy was associated with a 300% and 158% increase in myocardial and perivascular fibrosis, respectively. Cardiac transduction of the AT2R resulted in an 85% attenuation of LVWT, 91% attenuation of HW/BW, and a 43% decre...Continue Reading

References

Oct 1, 1991·The American Journal of Physiology·B F Cox, V S Bishop
Nov 1, 1984·The American Journal of Physiology·M K RaizadaC Sumners
Jun 1, 1996·Clinical and Experimental Pharmacology & Physiology·M J BlackC I Johnston
Oct 8, 1999·The Journal of Clinical Investigation·Y TsutsumiT Iwasaka
Mar 11, 2000·American Journal of Physiology. Endocrinology and Metabolism·S GallinatC Sumners
Mar 7, 2001·Hypertension·A S PachoriM K Raizada
Mar 9, 2002·Methods in Enzymology·Matthew J HuentelmanMohan K Raizada
May 23, 2002·Hypertension·Alok S PachoriMichael J Katovich
Sep 10, 2002·American Journal of Physiology. Renal Physiology·Susan P BagbyRobert C Speth
Dec 19, 2002·Physiological Genomics·Jason E ColemanMohan K Raizada
Mar 29, 2003·Hypertension Research : Official Journal of the Japanese Society of Hypertension·Hiroaki MukawaTakayuki Ito
Jul 23, 2003·Molecular and Cellular Biochemistry·Chihiro ShikataNobuakira Takeda
Feb 26, 2004·Hypertension·Yao Liang TangM Ian Phillips
Apr 21, 2004·Journal of Hypertension·Volkmar GrossFriedrich C Luft
May 29, 2004·Hypertension·Beverly L MetcalfeMohan K Raizada

❮ Previous
Next ❯

Citations

Jan 4, 2008·Naunyn-Schmiedeberg's Archives of Pharmacology·Robert C Speth, Vardan T Karamyan
Dec 14, 2005·Journal of Nuclear Cardiology : Official Publication of the American Society of Nuclear Cardiology·Grace P ChenJames H Caldwell
Apr 5, 2008·Clinical and Experimental Pharmacology & Physiology·Gerard J Gaspard, Kishore B S Pasumarthi
Aug 26, 2010·The Journal of Pain : Official Journal of the American Pain Society·Dora PinhoAntónio Albino-Teixeira
Sep 23, 2008·Pharmacology & Therapeutics·Emma S JonesRobert E Widdop
Nov 27, 2007·Biochemical and Biophysical Research Communications·Abhijit DandapatJawahar L Mehta
Jan 11, 2005·Experimental Physiology·Michael J KatovichMohan K Raizada
Oct 16, 2009·The Journal of Gene Medicine·Yanfei QiMichael J Katovich
Sep 18, 2009·Journal of the Renin-angiotensin-aldosterone System : JRAAS·Heiko Funke-KaiserThomas Unger
Oct 29, 2009·Journal of the Renin-angiotensin-aldosterone System : JRAAS·Anthony J Balmforth
Oct 29, 2009·Journal of the Renin-angiotensin-aldosterone System : JRAAS·Catherine A Lemarié, Ernesto L Schiffrin
Dec 14, 2005·Hypertension·Mohan K Raizada, Shant Der Sarkissian
Feb 12, 2009·Journal of Korean Medical Science·Hyung Jin YoonUNKNOWN Progressive REnal disease and Medical Informatics and gEnomics Research (PREMIER) members
Sep 18, 2010·Journal of Hypertension·U Muscha SteckelingsThomas Unger
Jul 1, 2006·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Hongwei LiColin Sumners
Jun 22, 2006·Physiological Genomics·Carlos Díez-FreireMohan K Raizada
Nov 14, 2006·American Journal of Physiology. Heart and Circulatory Physiology·Justin L GrobeMichael J Katovich
Nov 7, 2006·American Journal of Physiology. Heart and Circulatory Physiology·Hongwei LiColin Sumners
Jan 8, 2008·American Journal of Physiology. Heart and Circulatory Physiology·Xinhua YanJames P Morgan
Oct 17, 2008·Physiological Reviews·Jennifer DavisJoseph M Metzger
Jul 1, 2016·Clinical Science·Bryna S M Chow, Terri J Allen
Sep 12, 2019·Hypertension Research : Official Journal of the Japanese Society of Hypertension·Amanda S M BessaCarlos H Castro
Aug 3, 2010·American Journal of Physiology. Heart and Circulatory Physiology·María A CostaMariela M Gironacci
Jul 30, 2020·PloS One·Natia Q KelmSham S Kakar

❮ Previous
Next ❯

Related Concepts

Related Feeds

Cardiomyopathy

Cardiomyopathy is a disease of the heart muscle, that can lead to muscular or electrical dysfunction of the heart. It is often an irreversible disease that is associated with a poor prognosis. There are different causes and classifications of cardiomyopathies. Here are the latest discoveries pertaining to this disease.

CREs: Gene & Cell Therapy

Gene and cell therapy advances have shown promising outcomes for several diseases. The role of cis-regulatory elements (CREs) is crucial in the design of gene therapy vectors. Here is the latest research on CREs in gene and cell therapy.

Cardiomegaly

Cardiomegaly, known as an enlarged heart, is a multifactorial disease with different pathophysiological mechanisms. Hypertension, pregnancy, exercise-induced and idiopathic causes are some mechanisms of cardiomegaly. Discover the latest research of cardiomegaly here.