Angiotensin II upregulates renin-angiotensin system in human isolated T lymphocytes

Regulatory Peptides
Mirella CoppoLoredana Poggesi

Abstract

This study was aimed at investigating the effects of Angiotensin (Ang) II stimulation on T lymphocytes mRNA expression of angiotensinogen (AGTN), angiotensin-converting enzyme (ACE) and AT1-receptor (R) and on ACE activity and Ang II content. The effects of Ang II stimulus were studied in lipopolysaccharide (LPS)-stimulated or not stimulated lymphocytes. mRNA expression for interferon-gamma (INF-gamma) was also studied to investigate whether a link between lymphocyte RAS and immunological function might occur. mRNAs for AGTN, ACE and AT1-R were obtained from peripheral blood of 18 healthy subjects and were quantified by real time quantitative transcriptase-polymerase chain reaction (PCR). ACE activity was assayed in cell pellets and supernatants by measuring the hippuric acid formation by high performance liquid chromatography (HPLC) and Ang II cell content was measured by radioimmunoassay (RIA) after HPLC separation. All determination were performed under baseline conditions and after the addition of 10(e- 13) M Ang II to LPS-stimulated or unstimulated lymphocytes. Ang II caused a significant upregulation of T subset lymphocytes gene expression of ACE and AT1-R and of INF gamma, and a marked increase in ACE activity and cell A...Continue Reading

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Citations

Jun 6, 2013·Current Hypertension Reports·Mieczysław LitwinLidia Gackowska
Jun 7, 2011·Molecular Immunology·João Luiz Silva-FilhoAna Acacia Sá Pinheiro
May 24, 2016·International Journal of Molecular Sciences·Annayya AroorRavi Nistala
Mar 11, 2017·Journal of the Renin-angiotensin-aldosterone System : JRAAS·Mirella CoppoMaria Boddi
Apr 18, 2019·Nature Reviews. Immunology·Grant R DrummondChristopher G Sobey
Dec 17, 2014·American Journal of Physiology. Renal Physiology·Kaori NarumiSadayoshi Ito
Dec 8, 2020·Clinical Science·Fiona J WarnerChandana B Herath
Feb 2, 2021·Frontiers in Immunology·Léder Leal XavierAndrea Wieck
Sep 28, 2021·The American Journal of the Medical Sciences·M CoppoM Boddi

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