Angular dependence of scaftering from Escherichia coli cells

Applied Optics
D A Cross, P Latimer

Abstract

The scattering of visible light from randomly oriented systems of heterogeneous E. coli cells in water was measured as a function of angle for 10-90 degrees . The cells are assumed to be prolate ellipsoids of revolution whose dimensions are independently measured. For these cultures the average cell volume and axial ratio is 0.6 micro(3) and 2.81, respectively. Using the measured dimensions, the Rayleigh-Debye approximation for the homogeneous ellipsoid was used to calculate theoretical scattering. A newly developed Rayleigh-Debye equation for a coated ellipsoid was also used. Both cell models lead to good theoryexperiment agreement, especially the coated ellipsoid model. The absolute position of the two observed scattering minima and the wavelength dependence of these positions is in excellent agreement with the theoretical predictions. The existence of the scattering minima in randomly oriented heterogeneous cultures of E. coli is attributed to the uniformity of their minor axes.

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Citations

Aug 28, 2015·Colloids and Surfaces. B, Biointerfaces·Jana Schwarz-LinekWilson C K Poon
Apr 10, 2013·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Anastasiya I KonokhovaValeri P Maltsev
Jan 18, 2005·Biophysical Journal·Jeremy D WilsonThomas H Foster
Jan 1, 1996·Annual Review of Physical Chemistry·R Richards-Kortum, E Sevick-Muraca
Mar 30, 1973·Experimental Cell Research·L S Cram, A Brunsting
Oct 20, 1991·Biotechnology and Bioengineering·R I JeprasT Atkinson
Oct 1, 1978·Applied Optics·P LatimerC Moore
Apr 15, 1979·Applied Optics·D S Wang, P W Barber
Mar 1, 1978·Applied Optics·P W Barber, D S Wang
May 26, 2017·Photosynthesis Research·Margaret Gwyn Latimer Govindjee
Jul 1, 1982·Applied Optics·P Latimer, F Wamble
Sep 1, 1988·Microbiological Reviews·A L Koch
Jul 1, 1992·Journal of Bacteriology·A L Koch, S Woeste

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