Angular range, sampling and noise considerations for inverse light scattering analysis of nuclear morphology

Journal of Biophotonics
Haoran ZhangAdam Wax

Abstract

In recent years, significant work has been devoted to the use of angle-resolved elastic scattering for the extraction of nuclear morphology in tissue. By treating the nucleus as a Mie scattering object, techniques such as angle-resolved low-coherence interferometry (a/LCI) have demonstrated substantial success in identifying nuclear alterations associated with dysplasia. Because optical biopsies are inherently noninvasive, only a small, discretized portion of the 4π scattering field can be collected from tissue, limiting the amount of information available for diagnostic purposes. In this work, we comprehensively characterize the diagnostic impact of variations in angular sampling, range and noise for inverse light scattering analysis of nuclear morphology, using a previously reported dataset from 40 patients undergoing a/LCI optical biopsy for cervical dysplasia. The results from this analysis are applied to a benchtop scanning a/LCI system which compromises angular range for wide-area scanning capability. This work will inform the design of next-generation optical biopsy probes by directing optical design towards parameters which offer the most diagnostic utility.

References

Apr 6, 2002·Journal of the Optical Society of America. A, Optics, Image Science, and Vision·Adam WaxMichael S Feld
Sep 9, 2006·Journal of Dairy Science·R A Laven, K R Lawrence
Aug 19, 2007·Nature Methods·Wonshik ChoiMichael S Feld
Oct 28, 2011·Journal of Biomedical Optics·Neil TerryAdam Wax
Mar 25, 2016·Journal of Biophotonics·Mirjam SchürmannChii J Chan
Nov 16, 2017·Optics Letters·Zachary A SteelmanAdam Wax
Feb 23, 2018·Applied Optics·Zachary A SteelmanAdam Wax
Sep 28, 2018·Biomedical Optics Express·Pablo EuguiBernhard Baumann

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