Anidulafungin increases the antibacterial activity of tigecycline in polymicrobial Candida albicans/Staphylococcus aureus biofilms on intraperitoneally implanted foreign bodies

The Journal of Antimicrobial Chemotherapy
Ona RogiersSoňa Kucharíková

Abstract

We aimed to establish a novel murine intra-abdominal foreign body infection model to study the activity of anidulafungin and tigecycline against dual species Candida albicans/Staphylococcus aureus biofilms. In vitro and in vivo single and dual species biofilms were developed inside serum-coated triple-lumen catheters placed in 24-well plates or implanted intraperitoneally in BALB/c mice. The effect of tigecycline and anidulafungin alone and in combination was tested using clinically relevant concentrations. Scanning electron microscopy was used to visualize the mature biofilm structure developed intraperitoneally. Flow cytometry was used to determine the immunological response upon infection. Immunoblot analysis allowed us to determine the effect of anidulafungin on poly-β-(1,6)-N-acetylglucosamine in in vitro-grown S. aureus biofilms. We determined the MIC, MBC and in vitro susceptibility profile for anidulafungin and tigecycline against C. albicans and S. aureus in mixed and single species biofilms. We demonstrated that anidulafungin acts synergistically when combined with tigecycline against in vivo intra-abdominal biofilms. Moreover, we reveal that anidulafungin reduces the abundance of S. aureus poly-β-(1,6)-N-acetylglucos...Continue Reading

References

Jan 16, 2004·Drugs·George G ZhanelDaryl J Hoban
Mar 1, 2007·Current Opinion in Critical Care·Stijn I BlotJan J De Waele
Apr 30, 2008·Antimicrobial Agents and Chemotherapy·Bharat DamleJames Dowell
Jun 12, 2009·Kidney International·Rhianna MilesDavid W Johnson
Jul 18, 2009·The Journal of Antimicrobial Chemotherapy·Fred C Tenover, Richard V Goering
Aug 6, 2009·Journal of Chemotherapy·F Menichetti, G Sganga
Jan 30, 2013·Diagnostic Microbiology and Infectious Disease·Gary E Stein, Timothy Babinchak
Apr 3, 2013·Infection and Immunity·Brian M Peters, Mairi C Noverr
Aug 29, 2013·Current Opinion in Infectious Diseases·Mauricio Rebolledo, Juan C Sarria
Nov 26, 2014·Infection and Immunity·Eric F KongMary Ann Jabra-Rizk
Dec 19, 2014·Peritoneal Dialysis International : Journal of the International Society for Peritoneal Dialysis·Selma TobudicHeinz Burgmann
Jul 21, 2015·Annals of the New York Academy of Sciences·David S Perlin
Nov 4, 2015·Peritoneal Dialysis International : Journal of the International Society for Peritoneal Dialysis·Robiyanto RobiyantoRahul P Patel
Jan 6, 2018·The Journal of Antimicrobial Chemotherapy·Justin C BaderPaul G Ambrose

❮ Previous
Next ❯

Citations

Oct 20, 2018·PLoS Pathogens·Jurgen WuytsMichelle Holtappels
May 24, 2019·Pathogens·Wibke KrügerMaria Joanna Niemiec
Oct 18, 2019·Frontiers in Microbiology·Hans CarolusPatrick Van Dijck
Dec 22, 2019·Journal of Fungi·Maria Elisa RodriguesCélia F Rodrigues
Oct 31, 2020·Frontiers in Cellular and Infection Microbiology·Lolita RoisinFrançoise Botterel
Mar 7, 2021·Microorganisms·Katrien Van DyckPatrick Van Dijck
May 19, 2021·Critical Reviews in Microbiology·Fazlurrahman KhanYoung-Mog Kim
Aug 6, 2021·Journal of Basic Microbiology·Saraswathi NagarajShoba Narayan

❮ Previous
Next ❯

Related Concepts

Related Feeds

Antifungals (ASM)

An antifungal, also known as an antimycotic medication, is a pharmaceutical fungicide or fungistatic used to treat and prevent mycosis such as athlete's foot, ringworm, candidiasis, cryptococcal meningitis, and others. Discover the latest research on antifungals here.

CRISPR & Staphylococcus

CRISPR-Cas system enables the editing of genes to create or correct mutations. Staphylococci are associated with life-threatening infections in hospitals, as well as the community. Here is the latest research on how CRISPR-Cas system can be used for treatment of Staphylococcal infections.

Candida albicans

Candida albicans is an opportunistic, fungal pathogen of humans that frequently causes superficial infections of oral and vaginal mucosal surfaces of debilitated and susceptible individuals. Discover the latest research on Candida albicans here.

Biofilm & Infectious Disease

Biofilm formation is a key virulence factor for a wide range of microorganisms that cause chronic infections.Here is the latest research on biofilm and infectious diseases.

Candidiasis

Candidiasis is a common fungal infection caused by Candida and it can affect many parts for the body including mucosal membranes as well as the gastrointestinal, urinary, and respiratory tracts. Here is the latest research.

Candidiasis (ASM)

Candidiasis is a common fungal infection caused by Candida and it can affect many parts for the body including mucosal membranes as well as the gastrointestinal, urinary, and respiratory tracts. Here is the latest research.

Antifungals

An antifungal, also known as an antimycotic medication, is a pharmaceutical fungicide or fungistatic used to treat and prevent mycosis such as athlete's foot, ringworm, candidiasis, cryptococcal meningitis, and others. Discover the latest research on antifungals here.