PMID: 9159116May 27, 1997Paper

Animal fatty acid synthase: functional mapping and cloning and expression of the domain I constituent activities

Proceedings of the National Academy of Sciences of the United States of America
S S ChiralaSalih J Wakil

Abstract

Animal fatty acid synthase (FAS; EC 2.3.1.85) is a homodimer of a multifunctional subunit protein and catalyzes the synthesis of palmitate from acetyl-CoA, malonyl-CoA, and NADPH. The subunit (Mr approximately 270,000) carries seven distinct component activities and a site for the prosthetic group 4'-phosphopantetheine (acyl carrier protein). Based on proteolytic mapping, the organization of the activity domains along the subunit polypeptide from the N terminus is as follows: beta-ketoacyl synthase, acetyl and malonyl transacylases, beta-hydroxyacyl dehydratase, enoyl reductase, beta-ketoacyl reductase, acyl carrier protein, and thioesterase. By comparing the amino acid sequences of the chicken, rat, and human synthases, we found that kallikrein cleavage sites occur in the least conserved regions of the FAS polypeptide subunit. Determining the amino acid sequences of the N-terminal end of the major kallikrein cleavage peptides helped delineate the most likely boundaries of the component activities in the cDNA-derived amino acid sequence. To confirm this organization, we cloned the chicken FAS cDNA coding for domain I and expressed it in Escherichia coli as a maltose-binding fusion protein. The isolated recombinant protein conta...Continue Reading

References

Jan 1, 1975·Methods in Enzymology·M J Arslanian, S J Wakil
Sep 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·H TowbinJ Gordon
Jan 1, 1992·DNA Sequence : the Journal of DNA Sequencing and Mapping·K F BeckM Schweizer
Jun 15, 1991·European Journal of Biochemistry·A WitkowskiS Smith
Apr 1, 1989·Archives of Biochemistry and Biophysics·W Y HuangS J Wakil
May 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·C M AmyS Smith
Jun 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·K P HolzerG G Hammes
Jan 1, 1983·Annual Review of Biochemistry·S J WakilV C Joshi
Sep 12, 1995·Proceedings of the National Academy of Sciences of the United States of America·A JayakumarS J Wakil
Oct 1, 1994·Archives of Biochemistry and Biophysics·W Y HuangS J Wakil
Dec 10, 1996·Proceedings of the National Academy of Sciences of the United States of America·A JayakumarS J Wakil

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Citations

Feb 25, 2005·Lipids·Subrahmanyam S Chirala, Salih J Wakil
Dec 1, 2001·Trends in Microbiology·D McDevitt, M Rosenberg
Aug 25, 2010·Quarterly Reviews of Biophysics·Timm MaierNenad Ban
Jan 16, 2008·Accounts of Chemical Research·Hao Lu, Peter J Tonge
Sep 18, 2008·International Journal of Obesity : Journal of the International Association for the Study of Obesity·G V Ronnett, S Aja
May 24, 2006·Bioscience, Biotechnology, and Biochemistry·Hiroshi Yoneyama, Ryoichi Katsumata
May 10, 2003·Proceedings of the National Academy of Sciences of the United States of America·Subrahmanyam S ChiralaSalih J Wakil
Mar 15, 2001·Proceedings of the National Academy of Sciences of the United States of America·S S ChiralaS J Wakil
Nov 14, 1997·Proceedings of the National Academy of Sciences of the United States of America·A JayakumarS J Wakil
Apr 11, 2007·The FEBS Journal·Carsten D RichterKira J Weissman
Aug 25, 2015·Frontiers in Microbiology·Sara E DiomandéJulien Brillard
Feb 6, 2010·Journal of Molecular Biology·Günter PappenbergerMarkus G Rudolph
Mar 20, 2010·Diabetes·Jose Manuel Fernandez-RealAntonio Vidal-Puig
Jan 28, 2017·Future Medicinal Chemistry·Thales KronenbergerVinicius Gonçalves Maltarollo
Apr 27, 2012·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Yan LiuSong Li
Aug 2, 2006·Angewandte Chemie·Franz von NussbaumDieter Häbich
May 16, 2000·The Journal of Biological Chemistry·A K DasA K Hajra
Mar 22, 2007·Journal of Enzyme Inhibition and Medicinal Chemistry·Ali AshekSeung J Cho
Jan 28, 2017·Future Medicinal Chemistry·Thales KronenbergerVinicius Gonçalves Maltarollo
Jan 9, 2017·Journal of Molecular Modeling·Xuyun YangYue Liu
Sep 5, 2020·European Journal of Medicinal Chemistry·Preeti RanaSrinivas Nanduri
Mar 13, 2001·Journal of Molecular Biology·X QiuA K Konstantinidis
Dec 31, 2002·Archives of Biochemistry and Biophysics·Arumugam JayakumarGary L Clayman
Jan 23, 2019·Chemical Research in Toxicology·Bang-Jau YouHong-Zin Lee

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