Animal model for infection with Listeria monocytogenes

Current Protocols in Immunology
D H BuschE G Pamer

Abstract

This unit describes methods for infecting mice with L. monocytogenes. Optimal media for growth and methods to maintain bacterial virulence by passage through mice are included. Methods for determining the severity of splenic and hepatic infection are detailed, with strategies for distinguishing innate from specific immune responses following L. monocytogenes infection. This infection induces MHC class I-restricted CD8(+) cytolytic T lymphocytes that clear infection and provide long-term immunity. This unit describes methods that can be used for in vitro expansion of L. monocytogenes-specific T cells.

References

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Citations

Jan 14, 2011·Clinical and Vaccine Immunology : CVI·Yuelan YinXinan Jiao
Jun 29, 2010·Blood·Huiyuan ZhangStephanie S Watowich
Aug 24, 2011·Journal of Visualized Experiments : JoVE·Nancy WangThomas Brodnicki
Jun 28, 2015·The Journal of Immunology : Official Journal of the American Association of Immunologists·Sergey S SereginYasmina Laouar
Oct 13, 2015·European Journal of Immunology·Delphine GoubauCaetano Reis e Sousa

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