PMID: 9180909Aug 1, 1997Paper

Animal model of human medulloblastoma: clinical, magnetic resonance imaging, and histopathological findings after intra-cisternal injection of MHH-MED-1 cells into nude rats

Medical and Pediatric Oncology
M SchabetT Pietsch

Abstract

To establish an animal model of human medulloblastoma, we have injected human MHH-MED-1 cells into the cisterna magna of nude rats. Tumors grew in 3 out of 4 animals injected with 10(6) medulloblastoma cells. Affected animals showed little or no weight gain and eventually lost weight but did not develop obvious neurological symptoms until the end of observation on day 31 after inoculation. At this time, magnetic resonance imaging (MRI) in tumor-bearing rats revealed contrast enhancement in the region of the fourth ventricle and the cisterna magna. Neuropathological examination demonstrated corresponding leptomeningeal growth in the cisterna magna invading the medulla oblongata, and tumor growth within the fourth ventricle invading the pons. The tumors basically showed the same immunostaining pattern as MHH-MED-1 cells in vitro expressing neuron-specific enolase (NSE) and vimentin, but no neurofilaments (NFs), synapthophysin, or glial fibrillary acidic protein (GFAP). No tumor grew in the fourth animal, which had a normal weight gain and no alteration on MRI. In conclusion (1) the intrathecally injected human medulloblastoma cells spread similar to medulloblastomas in patients, (2) tumor growth is readily detected by MRI, (3) th...Continue Reading

References

Aug 15, 1977·International Journal of Cancer. Journal International Du Cancer·R M McAllisterM B Gardner
Nov 1, 1992·Journal of Neuro-oncology·J MartosK Voigt
Apr 15, 1992·Cancer·M B HazukaJ J Kinzie
Dec 1, 1991·Neurologia Medico-chirurgica·M YamadaT Hayakawa
Aug 1, 1991·Journal of Neuro-oncology·H S FriedmanD D Bigner
Jan 1, 1989·Journal of Neuropathology and Experimental Neurology·X M HeD D Bigner
May 1, 1989·Carcinogenesis·M R StrattonC S Cooper
Sep 1, 1985·Journal of Neuropathology and Experimental Neurology·P F JacobsenJ M Papadimitriou
Nov 1, 1981·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·S M Hsu, L Raine

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Citations

Nov 12, 2005·Clinical & Experimental Metastasis·Karen M GauvainKatherine Weilbaecher
Apr 12, 2007·Neurological Research·Atsuko ShoTakashi Watanabe
May 17, 2002·Veterinary Pathology·Q WuB S Carson
Aug 11, 2006·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Qin ShuXiao-Nan Li

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