PMID: 2477Feb 2, 1976

Anion and amine uptake and uncoupling in submitochondrial particles

European Journal of Biochemistry
G F AzzoneRaffaele Colonna

Abstract

1. Unlike chloroplasts, submitochondrial particles are not uncoupled by nigericin + KCl or NH4Cl. Also the uncoupling effect of lipophilic anions is largely independent of the addition of weak bases. 2. Low concentrations of permeant anions cause a shift of the steady-state energy level rather than a cycle of energy utilization. The degree of inhibition of ATP synthesis by tetraphenylboron is larger than required for the uptake of the anion. 3. Lipophilic anions such as bromthymolblue, bromcresolpurple, and 8-anilino-1-napthalene sulphonate cause a pH-independent, 50% uncoupling in submitochondrial particles at concentrations of 3, 30 and 30 muM, respectively. The passive interaction of bromthymolblue and bromcresolpurple appears as a pH-dependent distribution between two pHases. ATP causes a pH-independent slight shift in the anion distribution, with negligible anion accumulation. 4. Addition of amines to energized submitochondrial particles results in two types of effects; uptake of amines and uncoupling. While in chloroplasts amine uptake and uncoupling are closely associated, this is not the case in submitochondrial particles. The uncoupling effect is observed only with lipophilic and not with hydrophilic amines, and the de...Continue Reading

References

Nov 1, 1970·Archives of Biochemistry and Biophysics·R E McCarty, C H Coleman
Aug 4, 1970·Biochimica Et Biophysica Acta·E A Liberman, V P Skulachev
May 1, 1969·Biochimica Et Biophysica Acta·R O ChristiansenE RACKER
Feb 1, 1969·Proceedings of the National Academy of Sciences of the United States of America·A AzziC P Lee
Dec 31, 1973·Biochimica Et Biophysica Acta·G F Azzone, S Massari
Nov 15, 1972·FEBS Letters·G F Azzone, S Massari
Jan 18, 1973·Biochimica Et Biophysica Acta·S PapaD Larosa
Feb 1, 1974·FEBS Letters·P Dell'Antone, G F Azzone
Jul 1, 1972·Archives of Biochemistry and Biophysics·Raffaele ColonnaG Felice Azzone
Aug 9, 1972·Biochimica Et Biophysica Acta·D W DeamerA R Crofts
May 1, 1966·Proceedings of the National Academy of Sciences of the United States of America·B Chance, L Mela
Feb 1, 1970·European Journal of Biochemistry·E Rossi, G F Azzone
Mar 1, 1968·European Journal of Biochemistry·P MitchellL Smith
Dec 1, 1968·Archives of Biochemistry and Biophysics·A A Horton, L Packer
Nov 1, 1968·European Journal of Biochemistry·G F AzzoneS Massari
Sep 18, 1970·FEBS Letters·Raffaele ColonnaLuciano Pregnolato
Jun 3, 1960·Biochimica Et Biophysica Acta·N E GOOD
Sep 15, 1962·Proceedings of the National Academy of Sciences of the United States of America·E RACKER
May 1, 1959·Plant Physiology·D W KrogmannM Avron
Dec 1, 1970·The Journal of Membrane Biology·M MontalC P Lee

Citations

Dec 1, 1978·Archives of Biochemistry and Biophysics·P Dell'AntoneO Volpato

Related Concepts

Amines
Ammonia
Anions
Antibiotics
Bos indicus
Organic Pigments
Hydrogen-Ion Concentration
Mitochondria, Muscle
Myocardium
Pandavir

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