Anion channels in a leaky epithelium. A patch-clamp study of choroid plexus.

Pflügers Archiv : European journal of physiology
O ChristensenT Randlev

Abstract

We have used the patch-clamp technique to characterize three anion channels in the ventricular membrane of the choroid plexus epithelium from Necturus. The most frequently occurring channel had a nonlinear IV-curve. The conductance in excised patches with 112 mM chloride at both sides was 28 pS at 0 mV, increasing towards positive membrane potentials. The selectivity ratios were PNa:PCl less than or equal to 0.1 and PNO3:PCl:PHCO3 = 1.6:1:0.43. SITS and furosemide (1 mM) on the inside reduces chloride flux to 0.15 and 0.37 times the control value. In attached patches, the most commonly observed channel had a conductance of 7.5 pS. The single-channel current for this channel reversed direction at 15 mV hyperpolarization, indicating accumulation of chloride to a factor of 1.8 above equilibrium. External stimulation of the tissue by theophylline, IBMX and dbcAMP, or by hypotonic shock did not increase the activity of this channel. In very few excised patches, we have observed a chloride channel with a conductance of 7 pS with 112 mM chloride at both sides. The 7 pS channel appears to be identical to a 2 pS channel found in attached patches. The 2 pS channel was not normally active in attached patches but was activated in 28% of th...Continue Reading

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Citations

Nov 1, 1992·Pflügers Archiv : European journal of physiology·A Coulombe, E Coraboeuf
Jan 1, 1992·Pflügers Archiv : European journal of physiology·F BecqE Hollande
Jan 1, 1993·Journal of Inherited Metabolic Disease·M B Segal
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Sep 22, 2007·Cerebrospinal Fluid Research·Ian D MillarPeter D Brown
Mar 1, 1993·Pflügers Archiv : European journal of physiology·M KansenJ Bijman
Dec 11, 1997·The Journal of Experimental Zoology·E K Hoffmann
Jan 6, 2004·Fundamental & Clinical Pharmacology·Alexandra d'Anglemont de TassignyAlain Berdeaux
Jan 3, 2001·Microscopy Research and Technique·M B Segal
Dec 1, 1994·The American Journal of Physiology·R F KeepA L Betz
Oct 1, 1995·Kidney International·K Strange, P S Jackson

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