Anion channels, including ClC-3, are required for normal neutrophil oxidative function, phagocytosis, and transendothelial migration

The Journal of Biological Chemistry
Jessica G MorelandFred S Lamb

Abstract

NADPH oxidase activity, phagocytosis, and cell migration are essential functions of polymorphonuclear leukocytes (PMNs) in host defense. The cytoskeletal reorganization necessary to perform these functions has been extensively studied, but the role of cell volume regulation, which is likely dependent upon anion channels, has not been defined. Mice lacking the anion channel ClC-3 (Clcn3(-/-)) died from presumed sepsis following intravascular catheter placement, whereas Clcn3(+/+) littermates survived. We hypothesized that ClC-3 has a critical role in host defense and reasoned that PMN function would be compromised in these mice. Clcn3(-/-) PMNs displayed markedly reduced NADPH oxidase activity in response to opsonized zymosan and modestly reduced activity after phorbol 12-myristate 13-acetate. Human PMNs treated with the anion channel inhibitors niflumic acid or 5-nitro-2-(3-phenylpropylamino)benzoic acid had a very similar defect. ClC-3 protein was detected in the secretory vesicles and secondary granules of resting PMNs and was up-regulated to the phagosomal membrane. Clcn3(-/-) PMNs and human PMNs lacking normal anion channel function both exhibited reduced uptake of opsonized zymosan at 1, 5, and 10 min in a synchronized pha...Continue Reading

References

Feb 1, 1990·The Journal of Clinical Investigation·K H Krause, M J Welsh
Aug 1, 1986·The Journal of General Physiology·L Simchowitz, P De Weer
Jan 1, 1968·Scandinavian Journal of Clinical and Laboratory Investigation·C E Mogensen
May 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·H M KorchakG Weissmann
Jul 1, 1981·Clinical Pharmacokinetics·L Helleberg
Jul 14, 1995·The Journal of Biological Chemistry·J B BurrittA J Jesaitis
May 1, 1995·Inflammation Research : Official Journal of the European Histamine Research Society ... [et Al.]·M Roch-ArveillerJ R Sorenson
Jul 1, 1993·The American Journal of Physiology·J S StoddardL Simchowitz
Jan 5, 1999·The Journal of Biological Chemistry·T FriedrichT J Jentsch
Oct 16, 1999·Proceedings of the Association of American Physicians·W M Nauseef
Nov 26, 1999·Circulation Research·M García-DuránA López-Farré
Mar 4, 2000·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·M WheelerR G Thurman
Mar 28, 2002·Physiological Reviews·Thomas J JentschAnselm A Zdebik
Apr 23, 2002·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Takehiko OguraNobuya Inagaki
May 9, 2002·American Journal of Physiology. Cell Physiology·Xinhua LiSteven A Weinman
Dec 23, 2003·The Journal of Immunology : Official Journal of the American Association of Immunologists·Jessica G MorelandJerrold P Weiss
Jan 6, 2004·Fundamental & Clinical Pharmacology·Alexandra d'Anglemont de TassignyAlain Berdeaux
May 22, 2004·The Journal of Immunology : Official Journal of the American Association of Immunologists·Patricia Perez-CornejoPhilip A Knauf
Sep 21, 2004·Nature Reviews. Microbiology·Ferric C Fang
Jul 22, 2005·Nature·Alessandra Picollo, Michael Pusch

❮ Previous
Next ❯

Citations

Oct 6, 2006·Pflügers Archiv : European journal of physiology·Albrecht SchwabChristian Stock
Oct 24, 2007·Pflügers Archiv : European journal of physiology·Linda SchneiderAlbrecht Schwab
Jan 6, 2010·Pflügers Archiv : European journal of physiology·Leigh WellhauserChristine E Bear
Oct 7, 2009·Proceedings of the National Academy of Sciences of the United States of America·Deri MorganThomas E DeCoursey
Nov 21, 2009·The Journal of Biological Chemistry·James J MatsudaFred S Lamb
Jan 12, 2012·The Journal of Biological Chemistry·Fred S LambJessica G Moreland
Oct 9, 2008·The Journal of Biological Chemistry·A Paige Davis VolkJessica G Moreland
Oct 31, 2007·The Journal of General Physiology·Li-Ting Chien, H Criss Hartzell
Dec 17, 2008·Antioxidants & Redox Signaling·Fredrick D OakleyJohn F Engelhardt
Dec 9, 2008·Antioxidants & Redox Signaling·Aron B Fisher
Feb 12, 2009·Antioxidants & Redox Signaling·Fred S LambFrancis J Miller
May 15, 2009·Antioxidants & Redox Signaling·Thomas L LetoTakehiko Ueyama
Dec 24, 2011·TheScientificWorldJournal·Oliver Nüsse
Apr 10, 2009·American Journal of Physiology. Cell Physiology·Hiroe ShiratsuchiMarc D Basson
Mar 31, 2009·American Journal of Physiology. Lung Cellular and Molecular Physiology·Peter M Haggie, A S Verkman
Dec 20, 2011·Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology·Nathan D BozeatDayue Darrel Duan
Nov 13, 2010·Arteriosclerosis, Thrombosis, and Vascular Biology·Xi ChuFrancis J Miller
May 15, 2012·Circulation Research·Bernard LassègueKathy K Griendling
May 28, 2013·BMC Immunology·Xiang ZhuNives Zimmermann
Mar 21, 2008·Journal of Leukocyte Biology·Richard G PainterGuoshun Wang
Jan 22, 2010·Journal of Leukocyte Biology·Richard G PainterGuoshun Wang
Jan 15, 2011·Journal of Leukocyte Biology·David A WinkCarol A Colton
Jul 9, 2010·Journal of Leukocyte Biology·Ramona L McCaffreyLee-Ann H Allen
Aug 29, 2013·Vascular Health and Risk Management·Bruno K Rodiño-JaneiroEzequiel Alvarez
Jun 19, 2014·Nature Immunology·William M Nauseef, Niels Borregaard
Oct 25, 2012·Annual Review of Physiology·Tobias Stauber, Thomas J Jentsch
Sep 15, 2014·Apoptosis : an International Journal on Programmed Cell Death·Yun-Ying HuangGuan-Lei Wang
Jun 25, 2008·Seminars in Immunopathology·Balázs RadaErzsébet Ligeti
Feb 9, 2016·Autoimmunity Reviews·Anantha Maharasi RamaKrishnan, Kavitha Sankaranarayanan
Apr 14, 2010·FEBS Letters·Stefania AveraimoMichele Mazzanti
Feb 18, 2016·Oxidative Medicine and Cellular Longevity·Ana RamírezJavier Camacho
Sep 28, 2010·Free Radical Biology & Medicine·Ryan W BonvillainGuoshun Wang
Aug 30, 2008·Biochemical and Biophysical Research Communications·Jatinder Ahluwalia
Jan 1, 2008·Free Radical Biology & Medicine·Albert van der Vliet
Oct 12, 2007·The European Journal of Neuroscience·Guillaume DucharmeLyanne C Schlichter
Jan 5, 2011·British Journal of Pharmacology·Chihab KloseChristian Harteneck
Jan 28, 2012·Cardiovascular Therapeutics·Jennifer StreeterFrancis J Miller
May 23, 2014·Cellular Microbiology·William M Nauseef
Sep 23, 2014·Free Radical Biology & Medicine·Jessie N GreenChristine C Winterbourn

❮ Previous
Next ❯

Related Concepts

Related Feeds

ASBMB Publications

The American Society for Biochemistry and Molecular Biology (ASBMB) includes the Journal of Biological Chemistry, Molecular & Cellular Proteomics, and the Journal of Lipid Research. Discover the latest research from ASBMB here.

Bacterial Pneumonia

Bacterial pneumonia is a prevalent and costly infection that is a significant cause of morbidity and mortality in patients of all ages. Here is the latest research.

Bacterial Pneumonia (ASM)

Bacterial pneumonia is a prevalent and costly infection that is a significant cause of morbidity and mortality in patients of all ages. Here is the latest research.

Cell Migration

Cell migration is involved in a variety of physiological and pathological processes such as embryonic development, cancer metastasis, blood vessel formation and remoulding, tissue regeneration, immune surveillance and inflammation. Here is the latest research.