Mar 1, 1989

Anion channels with multiple conductance levels in a mouse B lymphocyte cell line

The Journal of Physiology
M M Bosma

Abstract

1. Multiple conductance level ion channels were recorded in excised and cell-attached patches from cells of a mouse B lymphocyte hybridoma line. The reversal potential for the single-channel current was unaffected by the species of cation on the cytoplasmic face of the patch, but changed as the Cl- concentration was altered, indicating that the channel is anion selective. 2. The permeability sequence determined from reversal potentials was F- greater than I- greater than SCN- greater than Br- greater than Cl- greater than glucuronate greater than NO3- greater than aspartate. This was different from the conductance sequence (Cl- greater than SCN- = F- greater than Br- greater than NO3- greater than I- greater than glucuronate greater than aspartate), indicating interaction of ions within the pore of the channel. Consistent with this was the observation of anomalous mole fraction dependence with a mixed solution of thiocyanate and chloride. 3. In addition to the main open level (about 400 pS; excised patch, symmetrical 165 mM-Cl-), three subconductance levels and one supraconductance level were observed. These were concluded to be integral components of the same channel based on coincidence of appearance and identical permeabilit...Continue Reading

  • References
  • Citations27

References

  • We're still populating references for this paper, please check back later.

Mentioned in this Paper

Chloride Ion Level
4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid
Nevus
Membrane
Talpidae
Aspartate
4-Acetamido-4'-isothiocyanatostilbene-2,2'-disulfonic Acid, Disodium Salt
Resting Potentials
Ions
Cyclic AMP

Related Feeds

Adhesion Molecules in Health and Disease

Cell adhesion molecules are a subset of cell adhesion proteins located on the cell surface involved in binding with other cells or with the extracellular matrix in the process called cell adhesion. In essence, cell adhesion molecules help cells stick to each other and to their surroundings. Cell adhesion is a crucial component in maintaining tissue structure and function. Discover the latest research on adhesion molecule and their role in health and disease here.