Mar 1, 1989

Anion channels with multiple conductance levels in a mouse B lymphocyte cell line

The Journal of Physiology
M M Bosma


1. Multiple conductance level ion channels were recorded in excised and cell-attached patches from cells of a mouse B lymphocyte hybridoma line. The reversal potential for the single-channel current was unaffected by the species of cation on the cytoplasmic face of the patch, but changed as the Cl- concentration was altered, indicating that the channel is anion selective. 2. The permeability sequence determined from reversal potentials was F- greater than I- greater than SCN- greater than Br- greater than Cl- greater than glucuronate greater than NO3- greater than aspartate. This was different from the conductance sequence (Cl- greater than SCN- = F- greater than Br- greater than NO3- greater than I- greater than glucuronate greater than aspartate), indicating interaction of ions within the pore of the channel. Consistent with this was the observation of anomalous mole fraction dependence with a mixed solution of thiocyanate and chloride. 3. In addition to the main open level (about 400 pS; excised patch, symmetrical 165 mM-Cl-), three subconductance levels and one supraconductance level were observed. These were concluded to be integral components of the same channel based on coincidence of appearance and identical permeabilit...Continue Reading

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Mentioned in this Paper

Chloride Ion Level
4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid
4-Acetamido-4'-isothiocyanatostilbene-2,2'-disulfonic Acid, Disodium Salt
Resting Potentials
Cyclic AMP

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