Anion secretion induced by capacitative Ca2+ entry through apical and basolateral membranes of cultured equine sweat gland epithelium

The Journal of Physiology
W H KoP Y Wong

Abstract

1. Anion secretion induced by capacitative Ca2+ entry through apical and basolateral membranes of cultured equine sweat gland epithelium was studied using the short-circuit current (Isc) technique. 2. Thapsigargin induced an increase in Isc that could be inhibited when external Ca2+ was chelated by EGTA. 3. The inhibition of the thapsigargin-induced Isc could be reversed by re-addition of Ca2+ to apical or basolateral solutions. The magnitude of the reactivated Isc depended predominantly on basolateral Ca2+ concentration. 4. The magnitude of the reactivated Isc upon basolateral Ca2+ addition increased with the thapsigargin concentration, indicating its dependence on the emptied state of the Ca2+ store induced by thapsigargin. 5. The thapsigargin-induced Isc, as well as the Ca(2+)-dependent reactivation of Isc in EGTA-treated epithelia, was inhibitable by apical, but not basolateral, addition of flufenamate, and by basolateral addition of La3+. Other Ca2+ channel blockers, verapamil and nifedipine, had no effect when applied to either membrane. 6. The results suggest that thapsigargin-induced anion secretion by the equine sweat gland epithelial cells is crucially dependent upon the Ca2+ influx occurring primarily through the bas...Continue Reading

Citations

Nov 7, 2006·Veterinary Dermatology·David McEwan JenkinsonDouglas L Bovell
Jul 28, 2011·Journal of Cellular and Molecular Medicine·A Berna-ErroJ A Rosado
Jun 12, 2013·Veterinary Dermatology·Douglas L BovellWing H Ko
Mar 31, 2015·Dermatologic Therapy·Melİh AkyolSedat Özçelİk
Jul 6, 2004·The Journal of Biological Chemistry·Grace Gar-Lee YueWing-Hung Ko
Jan 30, 2010·Pflügers Archiv : European journal of physiology·Stefan Feske
Jan 10, 2002·The Journal of Biological Chemistry·Connie Hau-Yan Wong, Wing-hung Ko
Feb 8, 2021·Experimental Physiology·Michael I Lindinger, Amanda P Waller
Jul 17, 1999·Trends in Neurosciences·K Kiselyov, S Muallem

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