PMID: 3746251Aug 1, 1986Paper

Anion/anion exchange in human neutrophils

The Journal of General Physiology
L SimchowitzP De Weer

Abstract

Of the total one-way chloride fluxes (approximately 1.4 meq/liter cell water X min) in steady state human polymorphonuclear leukocytes bathed in 148 mM Cl media, approximately 70% behaves as self-exchange mediated by a nonselective anion carrier that is not inhibited by stilbene disulfonates. Five properties of this carrier-mediated exchange were investigated: substrate saturation is seen with respect to 36Cl influx as a function of the external Cl concentration [for normal-Cl cells, the apparent Km(Cl) is approximately 22 mM when Cl replaces para-amino-hippurate (PAH) and approximately 5 mM when Cl replaces glucuronate], and with respect to 36Cl efflux as a function of the concentration of internal Cl replacing PAH [apparent Km(Cl) congruent to 35 mM for cells bathed in 148 mM Cl]; there is trans stimulation of 36Cl influx by internal Cl (replacing PAH) with an apparent Km(Cl) congruent to 35 mM, and of 36Cl efflux by external Cl with an apparent Km(Cl) congruent to 22 mM (Cl replacing PAH) or approximately 5 mM (Cl replacing glucuronate); there is substrate competition between Cl and PAH, but the carrier appears devoid of affinity for glucuronate; influxes and effluxes mediated by the carrier are subject to competitive inhibi...Continue Reading

Citations

Jan 1, 1993·The Journal of Membrane Biology·L Simchowitz, S K Vogt
Feb 1, 1993·Neuroscience·L BettendorffE Schoffeniels
Jul 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·A E LindseyR R Kopito
Mar 30, 2001·The Journal of General Physiology·P De WeerR F Rakowski
Jan 1, 1994·The Journal of Clinical Investigation·C Fittschen, P M Henson
Mar 15, 1993·The Journal of Experimental Zoology·A M Zetino, L B Kirschner
Sep 1, 1992·Kidney International·A P HaynesA G Morgan
Aug 3, 1994·Biochimica Et Biophysica Acta·G MulliertM Castaing
Aug 26, 2016·Immunological Reviews·Guoshun Wang
Jun 1, 1991·Clinical Biochemistry·S GrinsteinO D Rotstein
Oct 18, 2000·The Journal of Immunology : Official Journal of the American Association of Immunologists·R MenegazziP Patriarca
Sep 1, 1989·The American Journal of Physiology·D RestrepoP A Knauf
Apr 1, 1988·The American Journal of Physiology·L Simchowitz

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