ANKRD49 inhibits etoposide-induced intrinsic apoptosis of GC-1 cells by modulating NF-κB signaling

Molecular and Cellular Biochemistry
Xia ZhouHai-long Wang

Abstract

Spermatogenesis is a complicated process that is tightly regulated by the well-coordinated expression of a series of genes in the testes. Ankyrin repeat domain-containing protein 49 (ANKRD49), an evolutionarily conserved protein highly expressed in the testes, is mainly found in spermatogonia, spermatocytes, and round spermatids. However, the exact function of ANKRD49 in spermatogenesis has remained elusive. In this study, we sought to investigate the role of ANKRD49 in apoptosis and determine the mechanism underlying this process in male germ cell-derived GC-1 cells. Nuclear staining with Hoechst 33258 and annexin V-FITC/PI, as well as analysis of caspase 3 activity, mitochondrial membrane potential, and apoptotic protein expression, showed that etoposide-induced apoptosis was attenuated by ANKRD49 overexpression but promoted by RNA interference-induced ANKRD49 knockdown. Furthermore, assessment of the levels of caspase 9, caspase 8, and proteins of the Bcl-2 family revealed ANKRD49 to be involved in an intrinsic apoptosis pathway. Examination of the subcellular distribution of the NF-κB p65 subunit after treatment with an NF-κB signaling inhibitor or p65 small interfering RNA demonstrated that ANKRD49 modulated etoposide-indu...Continue Reading

References

May 8, 2000·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·C G Print, K L Loveland
Mar 5, 2004·Biochimica Et Biophysica Acta·Juanita C SharpeRichard J Youle
Jul 12, 2007·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Toshiki TanakaZbigniew Darzynkiewicz
Feb 13, 2008·Cell·Matthew S Hayden, Sankar Ghosh
May 20, 2008·Molecular and Cellular Endocrinology·Marie-Claude Hofmann
Nov 6, 2009·Journal of Reproductive Immunology·Rakshamani TripathiChandrima Shaha
Apr 21, 2010·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Chandrima ShahaDurga Prasad Mishra
Jun 4, 2010·Molecular Human Reproduction·Verónica A CodeliaRicardo D Moreno
Feb 23, 2012·Apoptosis : an International Journal on Programmed Cell Death·Ankur MaheshwariRajnesh K Sharma
Mar 13, 2012·Biochimica Et Biophysica Acta·Joanna SzczepanowskaJerzy Duszynski
Apr 10, 2012·Apoptosis : an International Journal on Programmed Cell Death·Ximena Bustamante-MarínRicardo D Moreno
Jan 25, 2013·Nature Reviews. Cancer·Darren J Burgess
Jul 28, 2015·Biochimie·Bhupender KumarRameshwar N K Bamezai
Aug 11, 2015·Expert Reviews in Molecular Medicine·Sara CorreiaSílvia Socorro
Feb 24, 2016·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Jakob LauritsenGedske Daugaard
Apr 16, 2016·Molecular Medicine Reports·Ting DuanBing Yao
May 13, 2017·Cell Death and Differentiation·Olivier Julien, James A Wells
Jul 15, 2017·Scientific Reports·Almas R JumaBert De Groef
Nov 30, 2018·International Journal of Molecular Sciences·Cristina Trejo-SolísJulio Sotelo

❮ Previous
Next ❯

Methods Mentioned

BETA
flow cytometry
nuclear translocation

Software Mentioned

GraphPad
Prism

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis