ANLN-induced EZH2 upregulation promotes pancreatic cancer progression by mediating miR-218-5p/LASP1 signaling axis

Journal of Experimental & Clinical Cancer Research : CR
Anbin WangPing Bie

Abstract

Pancreatic cancer is a highly lethal malignancy with poor prognosis. Anillin (ANLN), an actin binding protein, is upregulated and plays an important role in many malignant tumors. However, the precise role of ANLN in pancreatic cancer remains unclear. The expression of ANLN and its association with pancreatic cancer patient survival were analyzed using an online database and confirmed by immunohistochemistry. The ANLN protein expression in pancreatic cancer cell lines was detected by Western blot. Cell proliferation, colony formation and transwell assays in vitro and in vivo tumor growth were used to determine the role of ANLN in pancreatic cancer. Gene expression microarray analysis and a series of in vitro assays were used to elucidate the mechanisms of ANLN regulating pancreatic cancer progression. We found that the ANLN expression was significantly upregulated in pancreatic cancer tissues and cell lines. The high expression of ANLN was associated with tumor size, tumor differentiation, TNM stage, lymph node metastasis, distant metastasis and poor prognosis in pancreatic cancer. ANLN downregulation significantly inhibited cell proliferation, colony formation, migration, invasion and tumorigenicity in nude mice. Meanwhile, we...Continue Reading

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Citations

Jun 21, 2020·Cancers·Nguyen Minh Tuan, Chang Hoon Lee
Jun 5, 2021·Head & Face Medicine·Bing WangZhong-Cheng Gong
Jun 27, 2021·Experimental Biology and Medicine·Xuyang HouDaming Li
Jul 3, 2021·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Abolfazl AkbariShahram Agah
Oct 8, 2021·Journal of Clinical Laboratory Analysis·Limin MiaoHua Yuan

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Methods Mentioned

BETA
transfection
protein assay
light microscopy
xenograft
electrophoresis
Chip

Software Mentioned

SPSS17
DAVID
Transcriptome Analysis Console
Quantity One
Expression Console
Targetscan

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