Annexin 2 is a phosphatidylinositol (4,5)-bisphosphate binding protein recruited to actin assembly sites at cellular membranes

Journal of Cell Science
Ursula RescherVolker Gerke

Abstract

Annexin 2 is a Ca(2+)-regulated membrane protein and an F-actin-binding protein enriched at actin assembly sites both, on the plasma membrane and on endosomal vesicles. Here, we identify annexin 2 as a phosphatidylinositol (4,5)-bisphosphate (PtdIns(4,5)P(2))-interacting protein, thereby explaining this specific membrane association. Using the pleckstrin-homology (PH) domain of phospholipase Cdelta1 fused to yellow fluorescent protein as a marker for PtdIns(4,5)P(2), we show that annexin 2 and its ligand p11 (S100A10) are targeted to sites of PtdIns(4,5)P(2) enrichment where F-actin accumulates. At the plasma membrane, adhesion of pedestal-forming enteropathogenic Escherichia coli induces a recruitment of 1-phosphatidylinositol-4-phosphate 5-kinase (PtdIns4P 5-kinase) and an enrichment of PtdIns(4,5)P(2) and annexin 2-p11 at sites of bacterial adhesion. Induction of PtdIns(4,5)P(2)-enriched ruffles and PtdIns(4,5)P(2)-positive, actin-coated vacuoles by Arf6-mediated activation of PtdIns4P 5-kinase also leads to a concomitant accumulation of the annexin 2-p11 complex and the PH domain. Binding studies with immobilized phosphoinositides and phosphoinositide-containing liposomes reveal that the purified annexin 2-p11 complex direc...Continue Reading

References

Nov 6, 1992·Science·C E Creutz
Feb 24, 1995·Science·C D'Souza-SchoreyP D Stahl
Aug 1, 1995·Molecular and Cellular Biochemistry·D M Waisman
Aug 1, 1996·The Journal of Cell Biology·H RadhakrishnaJ G Donaldson
Mar 24, 1999·The Journal of Cell Biology·S MukherjeeF R Maxfield
Aug 17, 1999·Current Opinion in Cell Biology·P Chavrier, B Goud
Sep 7, 2000·The Journal of Cell Biology·E B Babiychuk, A Draeger
Oct 14, 2000·Annual Review of Cell and Developmental Biology·D L GoosneyB B Finlay
Nov 9, 2000·Cell Biology International·A Lewit-BentleyV Gerke
Jan 9, 2001·Trends in Cell Biology·F Frischknecht, M Way
Jul 17, 2001·Current Opinion in Cell Biology·T F Martin
Aug 18, 2001·Current Biology : CB·C J MerrifieldS E Moss
Sep 6, 2001·The Journal of Cell Biology·F D BrownJ G Donaldson
Dec 18, 2001·The Journal of Biological Chemistry·Alison SkippenShamshad Cockcroft
Mar 28, 2002·Physiological Reviews·Volker Gerke, Stephen E Moss
Apr 16, 2003·Traffic·Mark A Lemmon

❮ Previous
Next ❯

Citations

Jul 20, 2007·Pflügers Archiv : European journal of physiology·Ursula Rescher, Volker Gerke
Dec 23, 2008·Nature Cell Biology·Joshua W ZielDavid R Sherwood
Jun 2, 2005·Nature Reviews. Molecular Cell Biology·Volker GerkeStephen E Moss
Jan 25, 2008·Nature Reviews. Molecular Cell Biology·Mark A Lemmon
Oct 24, 2008·Nature Reviews. Molecular Cell Biology·Ira Mellman, W James Nelson
Jul 9, 2011·The Journal of Biological Chemistry·Mallika Valapala, Jamboor K Vishwanatha
Aug 30, 2008·The Journal of Biological Chemistry·Judith AustermannVolker Gerke
Jan 7, 2005·Molecular Biology of the Cell·Sylvette Chasserot-GolazMarie-France Bader
Jul 10, 2009·Molecular Biology of the Cell·Ah-Lai LawMatthew J Hayes
May 18, 2011·Cold Spring Harbor Perspectives in Biology·Annette ShewanKeith Mostov
Jan 1, 2013·Acta Crystallographica. Section D, Biological Crystallography·Gabriel OzorowskiHartmut Luecke
Mar 26, 2010·Journal of Virology·Perdita BackesVolker Lohmann
Dec 28, 2007·Molecular and Cellular Biology·Stéphanie HeyraudDanielle Gulino-Debrac
Dec 12, 2007·Molecular and Cellular Biology·Marjo de GraauwBob van de Water
Oct 11, 2005·Annual Review of Cell and Developmental Biology·Verena Niggli
Apr 17, 2012·International Journal of Cell Biology·Adam G GrieveMatthew J Hayes
Mar 19, 2013·Virology Journal·Bishnupriya Bhattacharya, Polly Roy
Dec 7, 2013·PloS One·Masahiro NakamoriMasayasu Matsumoto
Jan 30, 2013·International Journal of Molecular Sciences·María Antonia LizarbeJavier Turnay
Jan 18, 2013·ISRN Neurology·Toshihiro Masaki
May 29, 2014·The Journal of Clinical Investigation·Roberto FerrareseMarkus Bredel
Apr 15, 2014·Journal of Controlled Release : Official Journal of the Controlled Release Society·Terrick AndeyMandip Singh
May 20, 2014·Journal of Cell Communication and Signaling·Jessica K HitchcockGeorgia Schäfer
Jan 24, 2013·PloS One·Marcin ChlystunStephen E Moss
Apr 22, 2009·Cellular and Molecular Life Sciences : CMLS·Katia MonastyrskayaAnnette Draeger
Sep 19, 2012·Cellular and Molecular Life Sciences : CMLS·Natalie Porat-ShliomRoberto Weigert
Jun 15, 2012·Molecular Membrane Biology·Joanna Bandorowicz-PikulaSlawomir Pikula
Apr 28, 2006·FEBS Letters·Verena GoebelerUrsula Rescher
Mar 12, 2015·Oncology Reports·Xiao-Heng XuYan-Qiu Song
Apr 23, 2009·Cellular Signalling·Thomas Grewal, Carlos Enrich
Apr 30, 2009·The International Journal of Biochemistry & Cell Biology·Dongmin ShaoMustafa B A Djamgoz
Mar 26, 2016·Frontiers in Immunology·Julie Guignot, Guy Tran Van Nhieu
Sep 4, 2007·Biochimica Et Biophysica Acta·Avinash ChanderDevendra G Naidu
Jul 21, 2004·Traffic·Matthew J HayesStephen E Moss
Nov 30, 2007·Journal of Cellular Biochemistry·Agnieszka Strzelecka-KiliszekJoanna Bandorowicz-Pikula
Nov 4, 2006·The EMBO Journal·Anna LorussoEvelina Chieregatti
Apr 28, 2006·Neuropathology and Applied Neurobiology·L TatenhorstW Paulus

❮ Previous
Next ❯

Related Concepts

Related Feeds

Actin-binding Proteins

Actin-binding proteins are a component of the actin cytoskeleton that play essential roles in cellular functions such as regulation of actin polymerization, maintenance of cell polarity, gene expression regulation, cell motility and many more functions. Discover the latest research on actin-binding proteins here.