Annexin A11 knockdown inhibits in vitro proliferation and enhances survival of Hca-F cell via Akt2/FoxO1 pathway and MMP-9 expression

Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie
Shuqing LiuMing-Zhong Sun

Abstract

Annexin A11 (Anxa11), a Ca(2+)-regulated phospholipid-binding protein, is involved in cell apoptosis, differentiation, vesicle trafficking, cancer progression and autoimmune diseases. Previous study from our group indicated that Anxa11 was associated with lymphatic metastatic potential of murine hepatocarcinoma cells. Herein, we investigated the effects and action mechanism of Anxa11 knockdown on in vitro cell proliferation and apoptosis of Hca-F, a murine hepatocarcinoma cell with∼75% lymph node metastatic potential. Real-time PCR and western blotting assays indicated that Anxa11 was significantly downregulated in monoclonal Anxa11-shRNA-transfected Hca-F cells. Anxa11 knockdown in Hca-F suppressed its in vitro proliferation and cell apoptosis capacities. Following Anxa11 knockdown in Hca-F cells, Bax/Bcl-2 expression level ratio, Akt2 and FoxO1 (pSer319) expression levels as well as MMP-9 mRNA and active MMP-9 protein levels were significantly elevated in Hca-F cells. In conclusion, Annexin A11 knockdown inhibits the in vitro proliferation and cell apoptosis of Hca-F cell via Akt2/FoxO1 and/or MMP-9 expression pathway. Anxa11 might play an important role in hepatocarcinoma cell invasion and metastasis and hepatocarcinoma mali...Continue Reading

References

Apr 1, 1995·Archives of Biochemistry and Biophysics·A MizutaniH Hidaka
Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
Mar 8, 2002·Cellular Signalling·Karleen M Nicholson, Neil G Anderson
Dec 12, 2003·Lancet·Josep M LlovetJordi Bruix
Feb 10, 2004·The Journal of Biological Chemistry·Shyra J GardaiPeter M Henson
Apr 3, 2004·Genome Biology·Stephen E Moss, Reg O Morgan
Jun 16, 2004·The Journal of Cell Biology·Alejandra TomasStephen E Moss
Jan 12, 2005·The Journal of Biological Chemistry·Meiguo Xin, Xingming Deng
Jun 2, 2005·Nature Reviews. Molecular Cell Biology·Volker GerkeStephen E Moss
Aug 30, 2005·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Louise H WilliamsAndrew H Sinclair
Dec 12, 2007·British Journal of Cancer·R DuncanG I Murray
Mar 13, 2009·Biochimie·Mahdieh SadidiEva L Feldman
Mar 29, 2011·Biochimica Et Biophysica Acta·Xinbo ZhangArun K Rishi
Mar 30, 2011·Current Drug Targets·María Monsalve, Yolanda Olmos
May 28, 2011·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Jun ZhangJianwu Tang
Mar 24, 2012·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Rong-Kuan LiJian-Wu Tang
Jul 3, 2013·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Jun ZhangJianwu Tang
Oct 29, 2013·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Chaoming ZhangXilong Xiao
Feb 11, 2014·Clinica Chimica Acta; International Journal of Clinical Chemistry·Jiasheng WangFrederick T Greenaway

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Citations

Aug 31, 2018·Journal of Cellular Biochemistry·Yu-J JiaoMing-B Xiao
Dec 20, 2018·International Journal of Molecular Sciences·Chien-Chung YangChuen-Mao Yang

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