Annexin A2-dependent actin bundling promotes secretory granule docking to the plasma membrane and exocytosis

The Journal of Cell Biology
Marion GabelSylvette Chasserot-Golaz

Abstract

Annexin A2, a calcium-, actin-, and lipid-binding protein involved in exocytosis, mediates the formation of lipid microdomains required for the structural and spatial organization of fusion sites at the plasma membrane. To understand how annexin A2 promotes this membrane remodeling, the involvement of cortical actin filaments in lipid domain organization was investigated. 3D electron tomography showed that cortical actin bundled by annexin A2 connected docked secretory granules to the plasma membrane and contributed to the formation of GM1-enriched lipid microdomains at the exocytotic sites in chromaffin cells. When an annexin A2 mutant with impaired actin filament-bundling activity was expressed, the formation of plasma membrane lipid microdomains and the number of exocytotic events were decreased and the fusion kinetics were slower, whereas the pharmacological activation of the intrinsic actin-bundling activity of endogenous annexin A2 had the opposite effects. Thus, annexin A2-induced actin bundling is apparently essential for generating active exocytotic sites.

References

Mar 5, 1996·Proceedings of the National Academy of Sciences of the United States of America·N VitaleM Vaughan
Jan 1, 1996·Journal of Structural Biology·J R KremerJ R McIntosh
Oct 20, 1999·The Journal of Cell Biology·P W JanesA I Magee
Nov 9, 2000·Cell Biology International·A Lewit-BentleyV Gerke
Nov 21, 2000·Journal of Neuroscience Methods·F SeguraR Borges
Jan 22, 2003·Nature Neuroscience·Sethuraman SankaranarayananTimothy A Ryan
Nov 18, 2003·Molecular Biology of the Cell·Stéphane GasmanMarie-France Bader
Mar 23, 2004·Molecular Biology of the Cell·Bridget S WilsonJanet M Oliver
Jul 16, 2004·Molecular and Cellular Biology·Barak RotblatJohn F Hancock
Jul 21, 2004·Journal of Computational Chemistry·Eric F PettersenThomas E Ferrin
Jan 7, 2005·Molecular Biology of the Cell·Sylvette Chasserot-GolazMarie-France Bader
Jun 2, 2005·Nature Reviews. Molecular Cell Biology·Volker GerkeStephen E Moss
Oct 19, 2005·The Journal of Biological Chemistry·Nikhil A GokhaleWonhwa Cho
Nov 18, 2005·Methods : a Companion to Methods in Enzymology·John F Hancock, Ian A Prior
Jan 13, 2006·Nature Chemical Biology·Ryan R FalseyLuke Whitesell
Apr 8, 2006·The EMBO Journal·Matthew J HayesStephen E Moss
Aug 12, 2006·The EMBO Journal·Ruud F ToonenMatthijs Verhage
Oct 13, 2006·Biochimica Et Biophysica Acta·Magali MalacombeStéphane Gasman
May 5, 2007·The Journal of Physiology·Thorsten Lang
Jan 23, 2009·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Khajak BerberianManfred Lindau
Nov 17, 2010·Cellular and Molecular Neurobiology·S Chasserot-GolazN Vitale
Mar 28, 2012·International Review of Cell and Molecular Biology·Luis M Gutiérrez
Jul 24, 2012·Neuropharmacology·Ana M SebastiãoJoaquim A Ribeiro
Oct 13, 2012·Nature·Reinhard Jahn, Dirk Fasshauer
Oct 24, 2012·Biological Chemistry·Gabriel OzorowskiHartmut Luecke
Dec 12, 2012·The International Journal of Biochemistry & Cell Biology·Cristina J Torregrosa-HetlandLuis M Gutiérrez
Feb 6, 2013·The Journal of Cell Biology·Vanesa M TomatisFrédéric A Meunier
Mar 23, 2013·International Journal of Molecular Sciences·Alamelu BharadwajDavid Waisman
Jul 19, 2013·The Journal of Biological Chemistry·Patrick DrückerVolker Gerke
Sep 26, 2013·Frontiers in Endocrinology·Mohamed Raafet AmmarNicolas Vitale
Oct 30, 2013·The Journal of Cell Biology·Sandrine Poëa-GuyonNicolas Morel
Dec 7, 2013·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Andreas PapadopulosFrederic A Meunier
Feb 25, 2014·Nature Communications·Hsueh-Cheng ChiangLing-Gang Wu

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Citations

Feb 16, 2016·Journal of Neurochemistry·Emeline TanguyNicolas Vitale
Apr 3, 2016·Journal of Neurochemistry·Marion Gabel, Sylvette Chasserot-Golaz
Jul 4, 2016·Journal of Autoimmunity·R WeissJ Chapman
Jul 14, 2016·Médecine sciences : M/S·Marion GabelSylvette Chasserot-Golaz
Aug 1, 2016·Trends in Neurosciences·Frédéric A Meunier, Luis M Gutiérrez
Jul 13, 2016·The Journal of Cell Biology·Seema SinhaAlissa M Weaver
Jun 15, 2018·International Journal of Molecular Sciences·Anna Holthenrich, Volker Gerke
Aug 2, 2017·Biology of the Cell·Florence NiedergangChristophe Lamaze
Aug 31, 2017·Pflügers Archiv : European journal of physiology·Fernando D Marengo, Ana M Cárdenas
Oct 4, 2018·Scientific Reports·David GrillVolker Gerke
May 20, 2017·Frontiers in Cellular Neuroscience·Arlek M González-JamettAna M Cárdenas
Jul 22, 2017·Pflügers Archiv : European journal of physiology·Luis M Gutiérrez, José Villanueva
Sep 30, 2017·Biology of the Cell·Stéphane Gasman, Nicolas Vitale
Sep 13, 2020·Cells·Marion GabelSylvette Chasserot-Golaz
Jul 16, 2020·Cancers·Laura StreitStéphane Gasman
Dec 9, 2020·Advances in Biological Regulation·Emeline TanguyNicolas Vitale
Oct 10, 2021·Journal of Cellular and Molecular Medicine·Murilo Ramos Rocha, Jose Andres Morgado-Diaz

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Methods Mentioned

BETA
gene knockdown
electron microscopy
light microscopy
transmission electron microscopy
pulled down
electron tomography
transfection

Software Mentioned

IMOD
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ICY
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UCSF Chimera
Visual Basic
ImageJ

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