Annexin A4 inhibits sulfatide-induced activation of coagulation factor XII

Journal of Thrombosis and Haemostasis : JTH
Moeka NakayamaKyoko Kojima-Aikawa

Abstract

Factor XII (FXII) is a plasma serine protease that initiates the intrinsic pathway of blood coagulation upon contact with anionic substances, such as the sulfated glycolipid sulfatide. Annexins (ANXs) have been implicated in the regulation of the blood coagulation reaction by binding to anionic surfaces composed of phospholipids and sulfated glycoconjugates, but their physiological importance is only partially understood. To test the hypothesis that ANXs are involved in suppressing the intrinsic pathway initiated by sulfatide, we examined the effect of eight recombinant ANX proteins on the intrinsic coagulation reaction and their sulfatide binding activities. Recombinant ANXs were prepared in Escherichia coli expression systems and their anticoagulant effects on the intrinsic pathway initiated by sulfatide were examined using plasma clotting assay and chromogenic assay. ANXA4 active sites were identified by alanine scanning and fold deletion in the core domain. We found that ANXA3, ANXA4, and ANXA5 strongly inhibited sulfatide-induced plasma coagulation. Wild-type and mutated ANXA4 were used to clarify the molecular mechanism involved in inhibition. ANXA4 inhibited sulfatide-induced auto-activation of FXII to FXIIa and the conv...Continue Reading

References

Feb 11, 1991·FEBS Letters·R H NewmanM J Crumpton
Jan 15, 1991·Thrombosis Research·J RömischN Heimburger
Aug 25, 1987·Biochemistry·T FunakoshiK Fujikawa
Dec 15, 1987·Biochemistry·T FunakoshiK Fujikawa
Feb 15, 1986·Thrombosis Research·S SchiffmanA D Retzios
Apr 1, 1980·Biochemistry·K FujikawaE W Davie
Jun 1, 1994·British Journal of Haematology·T KosterJ P Vandenbroucke
Dec 13, 1996·Biochimica Et Biophysica Acta·Y KushiS Handa
Feb 21, 1998·Biochemistry·K S ChoiD M Waisman
May 23, 1998·The Journal of Biological Chemistry·R IshitsukaI Matsumoto
Mar 21, 2001·Biochimica Et Biophysica Acta·H SohmaY Kuroki
Jun 3, 2004·Journal of Biochemistry·Michiru IdaKyoko Kojima-Aikawa
Jun 4, 2004·Thrombosis and Haemostasis·Junko MasudaIsamu Matsumoto
Dec 14, 2004·Biochimica Et Biophysica Acta·R O MorganM P Fernandez
Jul 13, 2005·The Journal of Experimental Medicine·Thomas RennéBernhard Nieswandt
Aug 4, 2006·Nucleic Acids Research·Jinn-Moon Yang, Chi-Hua Tung
Mar 7, 2007·Human Molecular Genetics·Nadia BogdanovaArseni Markoff
Mar 27, 2007·Cellular Microbiology·Daniel E Voth, Robert A Heinzen
Apr 5, 2007·Proceedings of the National Academy of Sciences of the United States of America·Christian KannemeierKlaus T Preissner
Mar 11, 2008·Thrombosis and Haemostasis·Prasenjit GuchhaitPerumal Thiagarajan
May 24, 2014·Arteriosclerosis, Thrombosis, and Vascular Biology·Marijke J E KuijpersJudith M E M Cosemans
Dec 9, 2014·The New England Journal of Medicine·Harry R BüllerUNKNOWN FXI-ASO TKA Investigators
Jun 2, 2015·Journal of Korean Medical Science·Jeong In YangHo Yeon Kim
Apr 5, 2016·Annals of Neurology·Sarah HoppChristoph Kleinschnitz
Jun 19, 2016·Biological Chemistry·Thomas GrewalCarles Rentero
Sep 7, 2016·Nature Communications·Linda LabbertonThomas Renné

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Aug 5, 2020·International Journal of Molecular Sciences·Keisuke KomatsuyaKohji Kasahara

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