Annexin II modulates volume-activated chloride currents in vascular endothelial cells.

The Journal of Biological Chemistry
B NiliusG Droogmans

Abstract

The membrane-associated, microfilament-binding protein annexin II is abundantly expressed in endothelial cells from calf pulmonary artery (CPAE cells). We have analyzed its role in the regulation of volume-activated chloride currents (ICl, vol) by loading the cells via the patch pipette with a peptide comprising the N-terminal 14 residues of annexin II. This sequence harbors the binding site for the intracellular annexin II ligand, p11, and the peptide interferes with the annexin II-p11 complex formation. Loading of a CPAE cell with this peptide caused a gradual decrease in the amplitude of ICl, vol during repetitive stimulations with a 28% hypotonic extracellular solution. This run down of the current was virtually absent in untreated cells and in cells that were loaded with a mutated 14-amino acid peptide, which has a single amino acid replacement known to result in a more than 1000 times reduced affinity for binding to p11. We conclude that annexin II-p11 complex formation is either directly or indirectly involved in the activation of ICl, vol in endothelial cells.

References

Sep 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·H TowbinJ Gordon
Nov 6, 1992·Science·C E Creutz
Feb 1, 1988·Pflügers Archiv : European journal of physiology·M Pusch, E Neher
Oct 1, 1987·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·J R Glenney
Nov 30, 1995·Nature·S E Moss
Oct 1, 1994·Pflügers Archiv : European journal of physiology·M OikeB Nilius
May 1, 1994·The Journal of General Physiology·B NiliusG Droogmans
Apr 12, 1994·Proceedings of the National Academy of Sciences of the United States of America·M OikeB Nilius
Oct 1, 1995·Kidney International·K Strange, P S Jackson
Oct 1, 1996·General Pharmacology·B NiliusG Droogmans

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Citations

Oct 19, 1999·Microscopy Research and Technique·J H Henson
Apr 5, 2008·Pflügers Archiv : European journal of physiology·Grazia TammaGiovanna Valenti
Jan 1, 1997·Progress in Biophysics and Molecular Biology·B NiliusG Droogmans
Nov 27, 2001·Pathophysiology : the Official Journal of the International Society for Pathophysiology·Alexander A. Mongin, Sergei N. Orlov
Dec 1, 1999·Trends in Cardiovascular Medicine·H M KangD M Waisman
Jun 2, 2005·Nature Reviews. Molecular Cell Biology·Volker GerkeStephen E Moss
Jun 12, 2012·Proceedings of the National Academy of Sciences of the United States of America·Patricia Perez-CornejoVictor Faundez
Mar 12, 2008·The Journal of Biological Chemistry·Deming GouLin Liu
Jun 12, 2003·Genome Research·Steven A FarberMarnie E Halpern
Apr 22, 2009·Cellular and Molecular Life Sciences : CMLS·Katia MonastyrskayaAnnette Draeger
Jan 8, 2016·Pflügers Archiv : European journal of physiology·Stine F PedersenBernd Nilius
Oct 12, 1999·The Journal of Physiology·D TrouetJ Eggermont
Jun 29, 2004·Muscle & Nerve·Stefan Probst-CousinDieter Heuss
Aug 17, 2010·European Journal of Cell Biology·Ksenia AstaninaRalf Jacob
Dec 25, 2007·Neuroscience·D KlimmeckF Möhrlen
Oct 11, 2014·British Journal of Pharmacology·Yidong LiuLodewijk V Dekker
Jan 8, 2015·Acta Physiologica·S F PedersenB Nilius
Jul 6, 2012·Neurological Research·Liping NingShouwei Yue
Feb 1, 2003·Acta Physiologica Scandinavica·B Nilius, G Droogmans
Mar 29, 2005·The American Journal of Pathology·Djordje GvericM Louise Cuzner
Mar 21, 1998·The Journal of Biological Chemistry·G KassamD M Waisman
Mar 23, 2013·International Journal of Molecular Sciences·Alamelu BharadwajDavid Waisman
Apr 25, 2001·The Journal of Biological Chemistry·K S ChoiD M Waisman
Oct 14, 2009·Laboratory Investigation; a Journal of Technical Methods and Pathology·Osamu KidoTooru Shimosegawa
Oct 26, 1999·Circulation Research·A I BarakatP F Davies
Apr 8, 2006·The EMBO Journal·Matthew J HayesStephen E Moss
Sep 20, 2005·The Journal of Biological Chemistry·Emmanuelle DonierJohn N Wood
Oct 19, 2005·The Journal of Biological Chemistry·Nikhil A GokhaleWonhwa Cho
Sep 21, 2006·The Journal of Biological Chemistry·Sandrine De SerannoChristian Delphin
Mar 28, 2002·Physiological Reviews·Volker Gerke, Stephen E Moss
Oct 27, 2017·American Journal of Physiology. Cell Physiology·Lindsay M HerbertNikki L Jernigan
Aug 25, 2000·The Journal of Biological Chemistry·R MuimoA Mehta
Oct 3, 2001·Physiological Reviews·B Nilius, G Droogmans
Apr 29, 2004·American Journal of Physiology. Renal Physiology·David B N LeeIrene L Hale
Apr 13, 1999·The American Journal of Physiology·P TrouvéD Charlemagne
Jun 27, 1997·Biochimica Et Biophysica Acta·V Gerke, S E Moss

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