Annexin-V binds to the intracellular part of the beta(5) integrin receptor subunit

Biochemical and Biophysical Research Communications
Mikkel H AndersenJ T Rasmussen

Abstract

Bovine lactadherin binds to the alpha(v)beta(3) and alpha(v)beta(5) integrins in an RGD-dependent manner and also to anionic phospholipids. During the affinity purification of lactadherin binding receptors, a 35-kDa protein persistently coeluted with the alpha(v)beta(5) integrin receptor. Subsequently, peptide mapping, amino acid sequencing, and mass spectrometry analysis identified this protein as bovine annexin-V. Annexin-V accompanied the integrin receptor eluted with either RGD peptide or with EDTA suggesting that annexin-V bound specifically to the alpha(v)beta(5) integrin. To further investigate this putative interaction of annexin-V with the alpha(v)beta(5) integrin receptor, human annexin-V and intracellular domains of the human alpha(v)beta(5) integrin subunits were used in ligand blotting assays. Radiolabeled annexin-V showed weak binding to the intracellular part of beta(5) integrin subunit. However, by adding the aminophospholipid, phosphatidyl serine, the interaction with the beta(5) cytoplasmic peptide was enhanced many fold. Furthermore, the interaction was shown to be independent of phosphorylation, as annexin-V bound to unphosphorylated beta(5) peptide at a similar level to the phosphorylated peptide. Since bin...Continue Reading

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Citations

Apr 10, 2010·Clinical & Developmental Immunology·Yi-Jun ZhouZhen-Juan He
Mar 13, 2018·Medicinal Research Reviews·Anna A RybczynskaAren van Waarde
Sep 19, 2003·Biomedical Chromatography : BMC·Tibor CserhátiAdam Echardt
Jul 22, 2006·The FEBS Journal·Anja WinterAndreas Hofmann
Apr 10, 2010·Scandinavian Journal of Clinical and Laboratory Investigation·Lise FalborgMichael Rehling
Dec 15, 2011·Plant Cell Reports·Sravan Kumar JamiP B Kirti
May 4, 2006·The Journal of Immunology : Official Journal of the American Association of Immunologists·Carlos LeonNeil E Reiner
Sep 14, 2019·Journal of Cell Science·Chen YuSilvia C Finnemann
Sep 14, 2020·Journal of Thrombosis and Haemostasis : JTH·Hiroshi DeguchiJohn H Griffin
May 29, 2003·Molecular Cell·Marina Cardó-VilaRenata Pasqualini

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