DOI: 10.1101/511683Jan 4, 2019Paper

Annotated bacterial chromosomes from frame-shift-corrected long read metagenomic data

BioRxiv : the Preprint Server for Biology
Krithika ArumugamRohan B.H. Williams


Background: Short-read sequencing technologies have long been the work-horse of microbiome analysis. Continuing technological advances are making the application of long-read sequencing to metagenomic samples increasingly feasible. Results: We demonstrate that whole bacterial chromosomes can be obtained from a complex community, by application of MinION sequencing to a sample from an EBPR bio-reactor, producing 6Gb of sequence that assembles in to multiple closed bacterial chromosomes. We provide a simple pipeline for processing such data, which includes a new approach to correcting erroneous frame-shifts. Conclusions: Advances in long read sequencing technology and corresponding algorithms will allow the routine extraction of whole chromosomes from environmental samples, providing a more detailed picture of individual members of a microbiome.

Related Concepts

Nucleic Acid Sequencing
Molecular Genetic Technique
Bio E
Cyclin-Dependent Kinase Inhibitor 2A, Isoform 4

Related Feeds

BioRxiv & MedRxiv Preprints

BioRxiv and MedRxiv are the preprint servers for biology and health sciences respectively, operated by Cold Spring Harbor Laboratory. Here are the latest preprint articles (which are not peer-reviewed) from BioRxiv and MedRxiv.