Annulus cells from more degenerated human discs show modified gene expression in 3D culture compared with expression in cells from healthier discs

The Spine Journal : Official Journal of the North American Spine Society
Helen E GruberEdward N Hanley

Abstract

Understanding gene expression patterns of disc cells in culture is important as we develop biologic therapies for disc degeneration. The objective of the present study was to determine if cells from more degenerated discs expressed different genes, or differed in their expression patterns, compared with patterns of cells from healthier discs. To determine if annulus cells from more degenerated discs expressed different gene expression patterns compared with patterns of cells from healthier discs using genome-wide analysis. Cells from human annulus tissue were grown in three-dimensional (3D) culture and their gene expression patterns analyzed with Affymetrix microarray analysis. Gene expression patterns of cells from more degenerated discs (Thompson Grades IV and V) were compared with patterns from cells from healthier discs (Thompson Grades I, II, and III). After approval by our human subjects institutional review board, annulus cells were obtained from lumbar discs of seven subjects with Thompson Grades I, II, or III and from five subjects with discs of Thompson Grades IV and V. Cells were grown in 3D culture for 2 weeks; 3D cultures were used because this microenvironment more closely mimics the in vivo condition. mRNA was ha...Continue Reading

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Citations

Jan 31, 2013·European Spine Journal : Official Publication of the European Spine Society, the European Spinal Deformity Society, and the European Section of the Cervical Spine Research Society·Li JinXudong Li
May 1, 2013·Biotechnic & Histochemistry : Official Publication of the Biological Stain Commission·H E GruberE N Hanley
Mar 5, 2013·The Spine Journal : Official Journal of the North American Spine Society·Tsung-Ting TsaiJong-Hwei S Pang
Jan 22, 2013·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Bin NingShang-You Yang
Mar 22, 2014·Journal of Cellular and Molecular Medicine·Emerson KrockLisbet Haglund
Apr 21, 2012·Biochemical and Biophysical Research Communications·Rosalyn D AbbottJames C Iatridis
Jan 23, 2016·PloS One·Guus G H van den AkkerTim J M Welting
Jun 13, 2012·Journal of Anatomy·Girish PattappaSibylle Grad
May 23, 2015·Reviews in the Neurosciences·Sara HanaeiNima Rezaei

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