Anoctamin 3: A Possible Link between Cluster Headache and Ca2+ Signaling

Brain Sciences
Caroline RanAndrea Carmine Belin

Abstract

Cluster headache is a severe primary headache characterized by extremely painful attacks of unilateral headache. Verapamil is commonly used as a prophylactic treatment with good effect. In order to search for new pathways involved in the pathophysiology of cluster headache, we analyzed genetic variants that were previously linked to verapamil response in migraine in a Swedish cluster headache case-control sample. We used TaqMan qPCR for genetic screening and performed a gene expression analysis on associated genes in patient-derived fibroblasts, and further investigated which reference genes were suitable for analysis in fibroblasts from cluster headache patients. We discovered a significant association between anoctamin3, a gene encoding a calcium-activated ion channel, and cluster headache. The association was not dependent on verapamil treatment since the associated variant, rs1531394, was also overrepresented in patients not using verapamil. No difference was found in the anoctamin3 gene expression between controls and patients. Also, we determined that TBP, IPO8 and PDHB were suitable reference genes in cluster headache fibroblasts. This finding is the first report of an association between a variant in a gene encoding an ...Continue Reading

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Sep 8, 2017·Cephalalgia : an International Journal of Headache

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Citations

Apr 27, 2020·The Journal of Headache and Pain·Emer O'ConnorManjit Matharu
Jul 15, 2021·Cephalalgia : an International Journal of Headache·Carmen FourierAndrea Carmine Belin

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Methods Mentioned

BETA
PCR
Genotyping
biopsy

Software Mentioned

7500
OEGE HWE calculator
Primer3
geNorm
Mfold
PS Power and Sample Size Calculator
GraphPad Prism
GraphPad
SNP2TFBS
Normfinder

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