Anoctamin Channels in Human Myometrium: A Novel Target for Tocolysis.

Reproductive Sciences
Jennifer DanielssonGeorge Gallos

Abstract

Spontaneous preterm labor leading to preterm birth is a significant obstetric problem leading to neonatal morbidity and mortality. Current tocolytics are not completely effective and novel targets may afford a therapeutic benefit. To determine whether the anoctamin (ANO) family, including the calcium-activated chloride channel ANO1, is present in pregnant human uterine smooth muscle (USM) and whether pharmacological and genetic modulation of ANO1 modulates USM contraction. Reverse transcription-polymerase chain reaction (RT-PCR), quantitative RT-PCR, and immunohistochemical staining were done to determine which members of the ANO family are expressed in human USM. Uterine smooth muscle strips were studied in an organ bath to determine whether ANO1 antagonists inhibit oxytocin-induced USM contractions. Anoctamin 1 small interfering RNA (siRNA) knockdown was performed to determine its effect on filamentous-/globular (F/G)-actin ratio, a measurement of actin polymerization's role in promoting smooth muscle contraction. Messenger RNA (mRNA) encoding all members of the ANO family (except ANO7) are expressed in pregnant USM tissue. Anoctamin 1 mRNA expression was decreased 15.2-fold in pregnant USM compared to nonpregnant. Anoctamin ...Continue Reading

References

Jan 1, 1995·American Journal of Obstetrics and Gynecology·D M SlaterP R Bennett
Jun 18, 2002·Laboratory Investigation; a Journal of Technical Methods and Pathology·Sara A CarneyDarlene Dixon
Mar 13, 2003·Journal of Muscle Research and Cell Motility·Roger C YoungPeisheng Zhang
Apr 12, 2005·American Journal of Physiology. Lung Cellular and Molecular Physiology·Carol A HirshmanCharles W Emala
Jan 18, 2007·Annals of Biomedical Engineering·William L Maner, Robert E Garfield
Mar 3, 2007·Annals of the New York Academy of Sciences·Anatoly ShmygolSteven Thornton
Jun 29, 2007·Seminars in Cell & Developmental Biology·Adam M BrainardSarah K England
Jul 31, 2007·Seminars in Cell & Developmental Biology·Robert E Garfield, William L Maner
Sep 23, 2008·Cell·Björn Christian SchroederLily Yeh Jan
Oct 13, 2009·The Journal of Biological Chemistry·Loretta FerreraLuis J V Galietta
Dec 8, 2009·Proceedings of the National Academy of Sciences of the United States of America·Fen HuangLily Yeh Jan
Feb 26, 2011·The Journal of Biological Chemistry·Amelia MazzoneGianrico Farrugia
Aug 10, 2011·BMC Molecular Biology·Kate E O'DriscollFiona C Britton
Aug 23, 2011·American Journal of Physiology. Heart and Circulatory Physiology·Candice Thomas-GatewoodJonathan H Jaggar
Oct 18, 2011·Experimental Physiology·Kenton M SandersSean M Ward
Sep 19, 2012·Proceedings of the National Academy of Sciences of the United States of America·Fen HuangJason R Rock
Oct 11, 2012·BMJ : British Medical Journal·David M HaasNicky J Welton
Dec 4, 2012·American Journal of Physiology. Lung Cellular and Molecular Physiology·George GallosCharles W Emala
Feb 23, 2013·Proceedings of the National Academy of Sciences of the United States of America·Adrian BritschgiMohamed Bentires-Alj
Jul 20, 2013·Molecular Cancer·Ifeoma UbbyFranco Pagani
Sep 3, 2013·American Journal of Physiology. Lung Cellular and Molecular Physiology·George GallosCharles W Emala
Mar 7, 2014·The Journal of Biological Chemistry·Anke BillL Alex Gaither
Apr 3, 2014·Physiological Reviews·Nicoletta Pedemonte, Luis J V Galietta
Jun 8, 2014·Canadian Journal of Anaesthesia = Journal Canadien D'anesthésie·Mrinalini BalkiJose C A Carvalho
Jun 15, 2014·American Journal of Obstetrics and Gynecology·Kyra BernsteinGeorge Gallos
Jul 25, 2014·EMBO Molecular Medicine·Conor McCloskeyAndrew M Blanks
Aug 19, 2014·Frontiers in Physiology·Ramón A LorcaSarah K England
Dec 3, 2014·Journal of Molecular Biology·Alessandra PicolloAlessio Accardi
Mar 5, 2015·Journal of Molecular and Cellular Cardiology·Bingxiang WangZhiqiang Qu
Jun 10, 2015·ELife·Kuai YuH Criss Hartzell
Jul 2, 2015·Molecular Pain·Jorge Baruch Pineda-FariasVinicio Granados-Soto
Dec 25, 2015·Science Translational Medicine·Lihua YingDavid N Cornfield
Jan 27, 2016·Pflügers Archiv : European journal of physiology·Uhtaek Oh, Jooyoung Jung
Feb 4, 2016·Nature Communications·Michael S NakazawaM Celeste Simon
Nov 23, 2016·Proceedings of the National Academy of Sciences of the United States of America·Neville P Bethel, Michael Grabe
Jan 25, 2017·American Journal of Respiratory Cell and Molecular Biology·Maya MikamiCharles W Emala

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Citations

Oct 18, 2018·Reproductive Sciences·Maria Rosa Maduro
Nov 21, 2019·American Journal of Physiology. Lung Cellular and Molecular Physiology·Jennifer DanielssonCharles W Emala
Nov 27, 2019·Biology of Reproduction·Xiaofeng MaSarah K England
Jun 26, 2018·Journal of Smooth Muscle Research = Nihon Heikatsukin Gakkai Kikanshi·Shunsuke HyugaGeorge Gallos
Mar 3, 2021·Reproductive Sciences·Kenichiro MotomuraNardhy Gomez-Lopez
Feb 24, 2021·The Journal of Physiological Sciences : JPS·Shunsuke HyugaGeorge Gallos

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