Anoikis is regulated by BCL-2-independent pathways in human prostate carcinoma cells

The Prostate
Victor M Bondar, David J McConkey

Abstract

Loss of contact with the extracellular matrix (ECM) triggers a specialized form of apoptosis known as "anoikis" in normal epithelial cells. Dependence on adhesion to ECM is often lost in transformed cells, and the degree of anchorage independence may vary in non-metastatic and metastatic cancer cells. BCL-2 oncoprotein overexpression correlates with the progression and metastases of prostate cancer. Materials and Methods We studied anoikis in suspension cultures of PC-3 and LNCaP prostate carcinoma cells selected for enhanced metastatic potential in vivo and in PC-3 and LNCaP cells stably transfected with BCL-2. Apoptosis-associated DNA fragmentation was measured by agarose gel electrophoresis and propidium iodide staining and flow cytometry. Expression of BCL-2 family polypeptides was determined by immunoblotting. Non-metastatic PC-3P cells were significantly more sensitive to anoikis than the metastatic PC-3 variants (PC-3M, PC-3M-PRO-4, and PC-3M-LN-4), but anoikis resistance did not correlate with metastatic potential in LNCaP-derived cell lines. Expression of BCL-2 was higher in metastatic PC-3 and LNCaP subclones compared to isogenic non-metastatic cells, but these levels were not affected by anoikis. Enforced overexpress...Continue Reading

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Citations

Mar 3, 2004·The Prostate·Anastasios Tahmatzopoulos, Natasha Kyprianou
May 28, 2004·Expert Opinion on Pharmacotherapy·Anastasios TahmatzopoulosNatasha Kyprianou
Feb 5, 2013·Cancer Letters·Scott JenningHector Biliran
Mar 5, 2013·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·Keith O'BrienLorraine O'Driscoll
Jan 26, 2006·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Menghestab Haile
Oct 28, 2004·Journal of Cell Science·Alessandra MarconiCarlo Pincelli

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