PMID: 702651Oct 1, 1978Paper

Anomalous behavior of bacteriophage lambda polypeptides in polyacrylamide gels: resolution, identification, and control of the lambda rex gene product

Journal of Virology
M Belfort

Abstract

The resolution of lambia proteins was compared on the two types of sodium dodecyl sulfate-polyacrylamide gels commonly in use. The two kinds of gel differ essentially in the ratio of the cross-linker, N'-N-bismethylene-acrylamide (bisacrylamide), to acrylamide monomer. Several lambda proteins migrate relatively more slowly in gels with high bisacrylamide/acrylamide ratios (HB gels) than in gels with low ratios, although the two types of gel are of roughly equivalent porosity. This effect is illustrated by a change in relative position of both the Rex and Int proteins, with apparent increases in molecular weight of about 8 and 15%, respectively, in the HB gels. This work confirms that like repressor and Int, the 28.5-kilodalton protein, identified as Rex on HB gels, is postively regulated by the lambdacII and cIII products and negatively controlled Cro. An intact y site is required for Rex and repressor expression after infection, whereas their synthesis in a lysogen is dependent upon a functional maintenance promoter, Prm.

References

Oct 15, 1976·Virology·N KatzirA B Oppenheim
Dec 1, 1976·Virology·A Oppenheim, A B Oppenheim
Jun 15, 1977·Virology·A OppenheimA B Oppenheim
Jan 1, 1974·Proceedings of the National Academy of Sciences of the United States of America·K Shimada, A Campbell
Oct 25, 1973·Journal of Molecular Biology·H EcholsA Honigman
Jan 1, 1973·Annual Review of Genetics·I Herskowitz
Jan 1, 1972·Annual Review of Genetics·H Echols
Oct 15, 1974·Journal of Molecular Biology·E J Siden, M Hayashi
Jan 1, 1973·Molecular & General Genetics : MGG·K K Mark, W Szybalski
Sep 1, 1971·Proceedings of the National Academy of Sciences of the United States of America·L Reichardt, A D Kaiser
Oct 1, 1972·Journal of Virology·G N Gussin, V Peterson
Sep 1, 1969·Virology·E R SignerM A Brunstetter
Jun 15, 1955·Proceedings of the National Academy of Sciences of the United States of America·S Benzer
Aug 1, 1967·Virology·A W HarrisL Siminovitch

❮ Previous
Next ❯

Citations

Dec 23, 1998·Proceedings of the National Academy of Sciences of the United States of America·H Engelberg-KulkaG Glaser
Mar 23, 2005·Canadian Journal of Microbiology·Sidney Hayes, Roderick A Slavcev
Nov 5, 1999·Annual Review of Microbiology·H Engelberg-Kulka, G Glaser
Jan 1, 1980·Molecular & General Genetics : MGG·V PirrottaA Walz
Apr 21, 2021·Current Genetics·Shirley WongRoderick A Slavcev
May 1, 1981·Journal of Virology·M Lieb, C Talland
Oct 1, 1986·Applied and Environmental Microbiology·M A PfannenstielK W Nickerson
Nov 1, 1979·Gene·E H Szybalski, W Szybalski

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Proceedings of the National Academy of Sciences of the United States of America
R P DottinM L Pearson
Proceedings of the National Academy of Sciences of the United States of America
W G SpiegelmanH Eisen
© 2021 Meta ULC. All rights reserved