Anopheles gambiae PRS1 modulates Plasmodium development at both midgut and salivary gland steps.

PloS One
Thomas ChertempsIsabelle Rosinski-Chupin

Abstract

Invasion of the mosquito salivary glands by Plasmodium is a critical step for malaria transmission. From a SAGE analysis, we previously identified several genes whose expression in salivary glands was regulated coincident with sporozoite invasion of salivary glands. To get insights into the consequences of these salivary gland responses, here we have studied one of the genes, PRS1 (Plasmodium responsive salivary 1), whose expression was upregulated in infected glands, using immunolocalization and functional inactivation approaches. PRS1 belongs to a novel insect superfamily of genes encoding proteins with DM9 repeat motifs of uncharacterized function. We show that PRS1 is induced in response to Plasmodium, not only in the salivary glands but also in the midgut, the other epithelial barrier that Plasmodium has to cross to develop in the mosquito. Furthermore, this induction is observed using either the rodent parasite Plasmodium berghei or the human pathogen Plasmodium falciparum. In the midgut, PRS1 overexpression is associated with a relocalization of the protein at the periphery of invaded cells. We also find that sporozoite invasion of salivary gland cells occurs sequentially and induces intra-cellular modifications that inc...Continue Reading

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Citations

Jun 28, 2013·Malaria Journal·Sreelakshmi K SreenivasamurthyT S Keshava Prasad
Mar 8, 2016·Molecular and Biochemical Parasitology·Wansika PhadungsilRudi Grams
Dec 3, 2015·Parasites & Vectors·Michael B Wells, Deborah J Andrew
Feb 26, 2011·Immunological Reviews·Stefanie Steinert, Elena A Levashina
May 30, 2015·Parasites & Vectors·Geovane Dias-LopesJose Batista De Jesus
Nov 28, 2013·Pharmaceuticals·Olivia PerwitasariRalph A Tripp
Oct 9, 2016·Parasitology Research·Shuohan ZhengZhiyue Lv
Jan 24, 2020·Proceedings of the National Academy of Sciences of the United States of America·Alvaro Molina-CruzCarolina Barillas-Mury
May 6, 2021·The Korean Journal of Parasitology·Wansika Phadungsil, Rudi Grams

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Methods Mentioned

BETA
confocal microscopy
PCR
fluorescence microscopy

Software Mentioned

CLUSTAL
Mobyle
Phylip

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