Another Breaker of the Wall: the Biological Function of the Usp45 Protein of Lactococcus lactis.

Applied and Environmental Microbiology
Jhonatan A Hernandez-ValdesOscar P Kuipers

Abstract

Lactococcus lactis is a Gram-positive bacterium that is widely used as a cell factory for the expression of heterologous proteins that are relevant in the pharmaceutical and nutraceutical fields. The signal peptide of the major secreted protein of L. lactis, Usp45, has been employed extensively in engineering strategies to secrete proteins of interest. However, the biological function of Usp45 has remained obscure despite more than 25 years of research. Studies on Usp45 homologs in other Gram-positive bacteria suggest that Usp45 may play a role in cell wall turnover processes. Here, we show the effect of inactivation and overexpression of the usp45 gene on L. lactis growth, phenotype, and cell division. Our results are in agreement with those obtained in streptococci and demonstrate that the L. lactis Usp45 protein is essential for proper cell division. We also show that the usp45 promoter is highly activated by galactose. Overall, our results indicate that Usp45 mediates cell separation, probably by acting as a peptidoglycan hydrolase.IMPORTANCE The cell wall, composed mainly of peptidoglycan, is key to maintaining the cell shape and protecting the cell from bursting. Peptidoglycan degradation by peptidoglycan hydrolysis and a...Continue Reading

References

Jan 1, 1995·Methods in Molecular Biology·H Holo, I F Nes
Jun 1, 1996·Journal of Bacteriology·P G de RuyterW M de Vos
May 27, 2003·Trends in Biochemical Sciences·Daniel J RigdenMichael Y Galperin
May 27, 2003·Trends in Biochemical Sciences·Alex Bateman, Neil D Rawlings
Jun 9, 2004·Applied and Environmental Microbiology·Carine HuardMarie-Pierre Chapot-Chartier
Dec 13, 2005·Microbiology and Molecular Biology Reviews : MMBR·Dirk-Jan Scheffers, Mariana G Pinho
Dec 1, 1993·Applied and Environmental Microbiology·P R Jensen, K Hammer
Oct 25, 2007·Journal of Molecular Microbiology and Biotechnology·Séverine LayecNathalie Leblond-Bourget
Feb 22, 2008·Nucleic Acids Research·Jimin PeiNick V Grishin
Oct 4, 2008·Genes & Development·Alexander Y Mitrophanov, Eduardo A Groisman
Oct 23, 2008·Nucleic Acids Research·Lars J JensenChristian von Mering
Jun 21, 2011·Journal of Bacteriology·Adrian D Land, Malcolm E Winkler
Jul 19, 2011·Applied and Environmental Microbiology·Joao P C PintoJan Kok
Oct 19, 2011·Proceedings of the National Academy of Sciences of the United States of America·Lok-To ShamMalcolm E Winkler
Oct 25, 2011·Applied and Environmental Microbiology·Anisha GoelDouwe Molenaar
Nov 8, 2011·Current Opinion in Microbiology·Tsuyoshi Uehara, Thomas G Bernhardt
Dec 14, 2011·Methods in Molecular Biology·Julio Villatoro-HernándezRoberto Montes-de-Oca-Luna
Jun 30, 2012·Nature Methods·Johannes SchindelinAlbert Cardona
Nov 6, 2012·Applied and Environmental Microbiology·Daphne T W Ng, Casim A Sarkar
Jan 24, 2014·Bioinformatics·Philip JonesSarah Hunter
May 9, 2014·Nature Communications·Sergio G BartualJuan A Hermoso
Sep 5, 2014·Microbial Cell Factories·Marie-Pierre Chapot-Chartier, Saulius Kulakauskas

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