Anoxia-induced suspended animation in budding yeast as an experimental paradigm for studying oxygen-regulated gene expression.

Eukaryotic Cell
Kin Chan, Mark B Roth

Abstract

A lack of oxygen can force many organisms to enter into recoverable hypometabolic states. To better understand how organisms cope with oxygen deprivation, our laboratory previously had shown that when challenged with anoxia, both the nematode Caenorhabditis elegans and embryos of the zebrafish Danio rerio enter into suspended animation, in which all life processes that can be observed by light microscopy reversibly halt pending the restoration of oxygen (P. A. Padilla and M. B. Roth, Proc. Natl. Acad. Sci. USA 98:7331-7335, 2001, and P. A. Padilla, T. G. Nystul, R. A. Zager, A. C. Johnson, and M. B. Roth, Mol. Biol. Cell 13:1473-1483, 2002). Here, we show that both sporulating and vegetative cells of the budding yeast Saccharomyces cerevisiae also enter into a similar state of suspended animation when made anoxic on a nonfermentable carbon source. Transcriptional profiling using cDNA microarrays and follow-on quantitative real-time PCR analysis revealed a relative derepression of aerobic metabolism genes in carbon monoxide (CO)-induced anoxia when compared to nitrogen (N(2)) gas-induced anoxia, which is consistent with the known oxygen-mimetic effects of CO. We also found that mutants deleted for components of the mitochondrial...Continue Reading

References

Mar 1, 1992·Microbiological Reviews·R S Zitomer, C V Lowry
Jul 1, 1990·Journal of Bacteriology·M A DrebotG C Johnston
Jun 1, 1974·Bacteriological Reviews·L H Hartwell
Jan 1, 1980·Molecular & General Genetics : MGG·G C Johnston, R A Singer
Jun 1, 1993·Microbiological Reviews·M Werner-WashburneR A Singer
Jun 11, 1996·Proceedings of the National Academy of Sciences of the United States of America·A P Schmitt, K McEntee
Mar 1, 1996·Molecular Microbiology·M Werner-WashburneV M Peck
Sep 3, 1996·Proceedings of the National Academy of Sciences of the United States of America·P W HochachkaS C Land
Apr 1, 1998·Bioinformatics·T L Bailey, M Gribskov
May 13, 1999·Proceedings of the National Academy of Sciences of the United States of America·K E KwastR O Poyton
Jul 3, 1999·Nature Genetics·S TavazoieG M Church
Aug 31, 2000·Critical Care Medicine·P Safar, P Kochanek
Dec 2, 2000·Nature Genetics·M PrimigR E Esposito
Dec 5, 2000·Molecular Biology of the Cell·A P GaschP O Brown
Feb 17, 2001·Molecular Biology of the Cell·H C CaustonR A Young
Jun 19, 2001·Proceedings of the National Academy of Sciences of the United States of America·P A Padilla, M B Roth
May 15, 2002·Antioxidants & Redox Signaling·Claude A Piantadosi
Jul 26, 2002·Nature·Guri GiaeverMark Johnston
Aug 3, 2002·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Roland H Wenger
Jun 20, 2003·Annals of the New York Academy of Sciences·Chuan Shen, Jo Anne Powell-Coffman
Feb 1, 1953·Journal of Cellular Physiology·A A ANDREASEN, T J B STIER
Nov 8, 2003·Science·Todd G NystulMark B Roth
May 27, 2004·The American Journal of Pathology·Carine Michiels
Jun 28, 2005·Nucleic Acids Research·André BoorsmaHarmen J Bussemaker
Aug 12, 2005·Cytogenetic and Genome Research·P Lesage, A L Todeschini
Feb 21, 2006·Respiratory Physiology & Neurobiology·Michael G Jonz, Colin A Nurse
Feb 28, 2006·Journal of Insect Physiology·Thomas A GorrPablo Wappner

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