PMID: 9191110Jun 1, 1997Paper

Anoxia regulates gene expression in the central nervous system of Drosophila melanogaster

Brain Research. Molecular Brain Research
E Ma, Gabriel G Haddad

Abstract

We took advantage of the Drosophila melanogaster's extraordinary resistance to anoxia to study the molecular mechanisms underlying this phenomenon. We analyzed mRNA expression of heat shock proteins (HSP) (HSP26 and HSP70), ubiquitins (UB) (UB3 and UB4), cytochrome oxidase I (COXI) and superoxide dismutase (SOD) using slot blot analysis. The expression of HSP genes, especially HSP70, was remarkably up-regulated (up to a thousand-fold) while those of UB4 and COXI were down-regulated (10-60%) in response to the anoxic stress. The expression of UB3 gene was up-regulated by 1.5x and the expression of SOD gene was not significantly affected. In response to heat shock stress, the expression of HSP genes increased by up to several thousand-fold, the expression of UB genes increased modestly (23-91%) but the expression of SOD and COXI genes was reduced by 25%. Furthermore, the expression patterns of HSP genes under anoxia and heat shock were clearly different. The expression of HSP genes peaked by 15 min into anoxia and then declined but stayed above baseline. In contrast, their expression increased as a function of time during heat exposure. From these results, we conclude that: (1) different forms of stress regulates gene expression ...Continue Reading

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Citations

Apr 9, 2013·Translational Stroke Research·Priti Azad, Gabriel G Haddad
Apr 14, 2000·Brain Research. Molecular Brain Research·A N HegdeJ H Schwartz
Jan 8, 1999·Brain Research. Molecular Brain Research·E MaG G Haddad
Jun 17, 2000·Progress in Neurobiology·K J BanasiakG G Haddad
Oct 6, 1999·Current Opinion in Genetics & Development·S T Crews, C M Fan
May 1, 2007·Antioxidants & Redox Signaling·Satoshi OgawaOsamu Hori
Sep 23, 2010·Proteome Science·Hualing LiChenggang Zhang
Mar 9, 2011·Journal of Insect Physiology·Guijun ZhangKenneth B Storey
Nov 16, 2010·Journal of Insect Physiology·M Robert MichaudDavid L Denlinger
Nov 5, 2005·Alcoholism, Clinical and Experimental Research·R B CowmeadowN S Atkinson
Oct 23, 2009·Annals of the New York Academy of Sciences·Priti Azad, Gabriel G Haddad
Nov 4, 2004·Journal of Molecular Biology·Mingi HongJunho Lee
Jan 5, 2002·The Neuroscientist : a Review Journal Bringing Neurobiology, Neurology and Psychiatry·G G Haddad, E Ma
Aug 6, 2005·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·M NickersonM Fleshner
Dec 7, 2007·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Howard M Prentice
Nov 24, 2001·The Journal of Biological Chemistry·Qiaofang ChenGabriel G Haddad
Jul 18, 2019·G3 : Genes - Genomes - Genetics·Jacob B CampbellJon F Harrison

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