Anoxia-reoxygenation versus ischemia in isolated rat lungs

The American Journal of Physiology
G ZhaoA B Fisher

Abstract

Oxidant generation in anoxia-reoxygenation and ischemia-reperfusion was compared in isolated rat lungs. Anoxia-reoxygenation was produced by N2 ventilation followed by O2 ventilation. After anoxia, lung ATP content was decreased by 59%. Oxygenated ischemia was produced by discontinuing perfusion while ventilation with O2 was maintained. With anoxia-reoxygenation, oxidant generation, evaluated by oxidation of dichlorodihydrofluorescein (H2DCF) to fluorescent dichlorofluorescein, increased 3.6-fold, lung thiobarbituric acid reactive substances (TBARS) increased 342%, conjugated dienes increased 285%, and protein carbonyl content increased 46%. Pretreatment of lungs with 100 microM allopurinol inhibited the reoxygenation-mediated increase in lung fluorescence by 75% and TBARS by 69%. Oxygenated ischemia resulted in an approximately eightfold increase in lung H2DCF oxidation and a fourfold increase in TBARS, but allopurinol had no effect. On the other hand, 100 microM diphenyliodonium (DPI) inhibited the ischemia-mediated increase in lung fluorescence by 69% and lung TBARS by 70%, but it had no effect on the increase with anoxia-reoxygenation. Therefore, both ischemia-reperfusion and anoxia-reoxygenation result in oxidant generatio...Continue Reading

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Citations

Aug 25, 2000·American Journal of Physiology. Lung Cellular and Molecular Physiology·M P MerkerA B Fisher
Jul 28, 2004·American Journal of Physiology. Heart and Circulatory Physiology·Jeffrey M Dodd-oDavid B Pearse
Jan 10, 2006·American Journal of Respiratory Cell and Molecular Biology·Yan WangAron B Fisher
Jul 25, 2006·American Journal of Physiology. Lung Cellular and Molecular Physiology·Minqing ZhaoVictor E Laubach
Dec 5, 2019·American Journal of Physiology. Lung Cellular and Molecular Physiology·Eric J CharlesVictor E Laubach
Feb 18, 2003·American Journal of Respiratory and Critical Care Medicine·Marc de PerrotShaf Keshavjee
Sep 14, 2010·American Journal of Physiology. Heart and Circulatory Physiology·Willem A den HengstAn L Moens
Sep 1, 2011·Molecular and Cellular Biochemistry·Bhavesh SojitraSanjay Kumar Banerjee
Oct 23, 2001·American Journal of Physiology. Heart and Circulatory Physiology·Z WeiA B Fisher
May 12, 2004·American Journal of Physiology. Lung Cellular and Molecular Physiology·Yan WangAron B Fisher
Nov 15, 2011·Annual Review of Physiology·Elizabeth A BrowningAron B Fisher
Sep 16, 2020·Nutrients·Francine M AlmeidaRogerio Pazetti
May 10, 2005·American Journal of Physiology. Lung Cellular and Molecular Physiology·Satoshi SaitoYoshihiro Minamiya
Mar 26, 2005·American Journal of Respiratory and Critical Care Medicine·Jason D Christie
Nov 11, 2005·American Journal of Physiology. Lung Cellular and Molecular Physiology·Qunwei ZhangAron B Fisher
Aug 16, 2008·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Jonathan JunVsevolod Y Polotsky
Jul 21, 2016·Respiratory Research·John Zagorski, Jeffrey A Kline
May 11, 2002·Critical Care Medicine·Aron B FisherYefim Manevich
Apr 15, 2021·Archivos de bronconeumología·Amaia OjangurenJorge Juan Olsina-Kissler

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