PMID: 9175609Mar 1, 1997Paper

Antagonism of mGlu receptors and potentiation of EPSCs at rat spinal motoneurones in vitro

Neuropharmacology
C Q CaoP M Headley

Abstract

The patch-clamp technique has been used to record synaptic responses, elicited by electrical stimulation of dorsal roots, in 28 single motoneurones of in vitro spinal cord preparations from neonate (P5 to P8) rats. The effects of (RS)-alpha-methyl-4-phosphonophenylglycine (MPPG) (200 microM), a potent antagonist at L-2-amino-4-phosphonobutanoate (AP4)-sensitive receptors, and (RS)-alpha-methyl-4-carboxyphenylglycine (MCPG) (500 microM), which is a less selective antagonist of mGluRs, were tested on EPSCs alone and as antagonists of AP4-induced depression of EPSCs. The EC50 for depression of EPSCs by AP4 (1.16 +/- 0.12 microM, n = 8) was increased to 18.9 +/- 0.7 microM (n = 6) by MPPG. MCPG (500 microM) had no significant effect on the depressant potency of AP4. Under control conditions, EPSCs had mean peak amplitudes of 983 pA +/- 64 SEM and mean charge transferred of 306 +/- 37 pC (n = 28). These values were increased significantly (p < 0.05) to 1168 +/- 68 pA and 363 +/- 39 pC by MPPG (n = 6), and 1150 +/- 54 pA and 358 +/- 33 pC (n = 6) by MCPG. There was no significant difference between the enhancement of the initial peak of the EPSCs (mean latency from stimulus artifact 5.9 +/- 0.3 ms) and later components, suggesting mG...Continue Reading

References

Dec 1, 1989·Journal of Neuroscience Methods·M G BlantonA R Kriegstein
Aug 1, 1981·Pflügers Archiv : European journal of physiology·O P HamillF J Sigworth
Jan 1, 1995·Neuropharmacology·J P Pin, R Duvoisin
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Citations

Feb 12, 1999·Brain Research. Brain Research Reviews·R Anwyl
Aug 26, 2014·Neurochemical Research·Marion S Mercier, David Lodge
Oct 26, 1999·Neuropharmacology·D D SchoeppJ A Monn
May 15, 2003·British Journal of Pharmacology·Patrick A Howson, David E Jane
May 10, 2008·Nature Reviews. Neuroscience·Paulo S Pinheiro, Christophe Mulle
Sep 24, 2004·The Journal of Pharmacology and Experimental Therapeutics·Victor L ArvanianLorne M Mendell
Apr 4, 2000·Physiological Reviews·J C ReklingJ L Feldman

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