Antagonistic activities of Rho and Rac GTPases underlie the transition from neural crest delamination to migration

Developmental Dynamics : an Official Publication of the American Association of Anatomists
Irit Shoval, Chaya Kalcheim

Abstract

Neural crest progenitors arise as epithelial cells and then undergo a transition into mesenchyme that generates motility. Previously, we showed that active Rho maintains crest cells in the epithelial conformation by keeping stress fibers and membrane-bound N-cadherin. While Rho disappears from cell membranes upon delamination, active Rac1 becomes apparent in lamellipodia of mesenchymal cells. Loss of Rac1 function at trunk levels inhibited NC migration but did not prevent cell emigration that is associated with N-cadherin downregulation and G1/S transition. Furthermore, inhibition of Rho stimulated premature Rac1 activity and consequent formation of lamellipodia, leading to NC migration. To examine whether timely migration influences cell fate, Rac1 activity was transiently inhibited to delay dispersion of early NC cells that generate neural derivatives, and its activity was restored by the time of melanoblast migration. Even if confronted with a melanocytic environment, late-dispersing progenitors colonized sensory ganglia where they generated neurons and glia. In the context of crest delamination and migration, activities of Rho and Rac are differential, sequential, and antagonistic. Furthermore, transient inhibition of Rac1 ...Continue Reading

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Citations

Dec 30, 2015·Journal of Clinical Medicine·Chaya Kalcheim
Oct 10, 2012·Development, Growth & Differentiation·Erez Nitzan, Chaya Kalcheim
Nov 21, 2015·Cellular and Molecular Life Sciences : CMLS·Alice Roycroft, Roberto Mayor
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Apr 21, 2018·Genesis : the Journal of Genetics and Development·Nadège GouignardEric Theveneau
Jan 26, 2018·Genesis : the Journal of Genetics and Development·Chaya Kalcheim
Aug 21, 2020·The Journal of Biological Chemistry·Martin J BakerMarcelo G Kazanietz
Jun 24, 2019·Cell Communication and Signaling : CCS·Deepak KumarChaya Kalcheim
Dec 28, 2017·Genesis : the Journal of Genetics and Development·Clarissa C Pasiliao, Sevan Hopyan
Jul 29, 2018·The Journal of Cell Biology·W Christopher RisherCagla Eroglu
Jul 14, 2020·Frontiers in Cell and Developmental Biology·Kwantae KimAlberto Stolfi
May 1, 2021·International Journal of Molecular Sciences·Dina Rekler, Chaya Kalcheim
Jul 11, 2021·Cells & Development·Meritxell Font-NogueraMagali Suzanne

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