Antagonistic Activity of Endophytic and Rhizosphere Fungi Isolated From Sea Purslane (Sesuvium portulacastrum ) Against Pythium Damping off of Cucumber

Plant Disease
Thamodini G KarunasingheA M Al-Sadi

Abstract

This study was conducted to investigate the antagonistic activity of endophytic and rhizosphere fungi isolated from a medicinal plant, Sesuvium portulacastrum, against Pythium aphanidermatum, the cause of damping off of cucumber. A total of 40 endophytic and 19 rhizosphere fungi were isolated from S. portulacastrum. Three endophytic isolates and two rhizosphere isolates gave >50% suppression of P. aphanidermatum in the in vitro dual-culture tests. Scanning electron microscopic studies at the inhibition zone showed hyphae wall damage and abnormal mycelial growth of the genus Pythium. Molecular analysis identified the antagonistic endophytes as Aspergillus insulicola (isolate A435), A. insulicola (A419), and Aspergillus melleus (A412) and the rhizosphere antagonists as Aspergillus terreus (A213) and Aspergillus luchuensis (A116). Except for A116, the culture filtrates of the other antagonists significantly increased the electrolyte leakage from Pythium mycelia, whereas ethyl acetate extracts of A435, A412, and A213 showed significant growth suppression. All five antagonists were able to produce varying amounts of cellulase and β-glucanase enzymes. However, A435, A412, and A213 showed significantly higher cellulase activity, where...Continue Reading

References

Oct 18, 2013·Molecular Biology and Evolution·Koichiro TamuraSudhir Kumar
May 25, 2016·International Journal of Food Microbiology·María Silvina Alaniz ZanonSofía Noemí Chulze
Feb 16, 2017·Journal of Natural Products·Huzefa A RajaNicholas H Oberlies
Aug 22, 2017·Frontiers in Microbiology·Elham A KazerooniAbdullah M Al-Sadi
Jan 24, 2019·Plant Disease·Zainab M Al-BalushiAbdullah M Al-Sadi
Jun 13, 2019·International Journal of Environmental Research and Public Health·Brenda Sánchez-MontesinosMila Santos
Nov 21, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Olumide Owolabi OmoboyeMonica Höfte

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Citations

Jun 3, 2021·Pathogens·Daniele Cristina FontanaDurval Dourado Neto

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