Antagonistic effect of CRP and KdgR in the transcription control of the Erwinia chrysanthemi pectinolysis genes

Molecular Microbiology
William NasserSylvie Reverchon

Abstract

The main virulence factors of the phytopathogenic bacteria Erwinia chrysanthemi are pectinases that cleave pectin, a major constituent of the plant cell wall. The cyclic AMP receptor protein (CRP) was identified as the main activator of the pectinolysis genes. Gel shift and DNase I footprinting experiments showed that the purified E. chrysanthemi CRP protein binds specifically to the promoter regions of seven pectinolysis genes (pelB, pelC, pelD, pelE, ogl, kduI and kdgT) whose expression is positively regulated in vivo by CRP. In contrast, no interaction was observed between CRP and the promoter-operator region of pelA, whose expression is negatively regulated in vivo by CRP. Primer extension experiments demonstrated that each of the pelB, pelC, pelE and kduI genes is expressed from a unique sigma70 promoter, whereas ogl and kdgT possess three and two functional promoters respectively. The position of the CRP binding site relative to the transcription start site suggests that CRP acts as a primary activator at the pelB (via the CRP binding site 1), pelC, pelE, pelD, kdgTP1 and oglP2 promoters. In contrast, transcription at the kduI, oglP1 promoters seems to require another transcriptional activator in synergy with CRP. Investi...Continue Reading

Citations

Jun 29, 2010·The Journal of Biological Chemistry·Wilfred D KepseuWilliam Nasser
Dec 16, 2005·Molecular Plant-microbe Interactions : MPMI·William NasserMartine Boccara
Apr 14, 2006·Molecular Plant-microbe Interactions : MPMI·Quan PengChing-Hong Yang
Nov 9, 2002·Molecular Plant-microbe Interactions : MPMI·Thierry FranzaDominique Expert
Mar 26, 2003·Molecular Plant-microbe Interactions : MPMI·Hiroyuki MatsumotoShinji Tsuyumu
Dec 26, 2006·Applied and Environmental Microbiology·Shihui YangChing-Hong Yang
Oct 14, 2006·BMC Genomics·Chern-Sing GohMark Gerstein
Feb 2, 1999·Molecular Plant-microbe Interactions : MPMI·T FranzaD Expert
Sep 29, 2006·Journal of Theoretical Biology·Jacques-A SepulchreWilliam Nasser
Dec 29, 2013·Trends in Microbiology·Alexandre DupreyWilliam Nasser
Feb 14, 2006·FEMS Microbiology Reviews·Antonio J Molina-HenaresJuan L Ramos
Jul 20, 2010·Molecular Plant-microbe Interactions : MPMI·Beth MoleSarah R Grant
Jan 5, 2002·The Journal of Biological Chemistry·Nicolas BlotGuy Condemine
Apr 11, 2019·Environmental Microbiology·Alexandre DupreySylvie Reverchon
Nov 6, 2004·Microbiology·Dmitry A RodionovNicole Hugouvieux-Cotte-Pattat
Sep 14, 2001·Molecular Microbiology·N Hugouvieux-Cotte-Pattat, S Reverchon

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