Antagonistic effects of transforming growth factor-beta on vitamin D3 enhancement of osteocalcin and osteopontin transcription: reduced interactions of vitamin D receptor/retinoid X receptor complexes with vitamin E response elements

Endocrinology
A StaalJ B Lian

Abstract

Osteocalcin and osteopontin are noncollagenous proteins secreted by osteoblasts and regulated by a complex interplay of systemic and locally produced factors, including growth factors and steroid hormones. We investigated the mechanism by which transforming growth factor-beta (TGF beta) inhibits 1,25-dihydroxyvitamin D3 (1,25-(OH)2D3)-enhanced expression of the osteocalcin (OC) and osteopontin (OP) genes. ROS 17/2.8 cells, in which both genes are expressed, were transfected with reporter constructs driven by native (i.e. wild-type) rat OC and mouse OP promoters. TGF beta abrogated the 1,25-(OH)2D3 enhanced transcription of both the OC and OP genes. The inhibitory TGF beta response for each requires vitamin D response element (VDRE) sequences, although there are additional contributions from proximal basal regulatory elements. These transcriptional effects were further investigated for contribution of the trans-activating factors, which interact with OC and OP VDREs, involving the vitamin D receptor (VDR) and retinoid X receptor (RXR). Gel mobility shift assays show that TGF beta significantly reduces induction of the heterodimers VDR/RXR complexes in 1,25-(OH)2D3-treated ROS 17/2.8 cells. However, Western blot and ligand bindin...Continue Reading

Citations

Apr 27, 2012·Current Osteoporosis Reports·Grażyna E Sroga, Deepak Vashishth
Feb 6, 2004·Proceedings of the National Academy of Sciences of the United States of America·Laura P Zanello, Anthony W Norman
Oct 5, 2001·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·A J BortonR S Weinstein
May 2, 2014·Frontiers in Physiology·Jeroen van de Peppel, Johannes P T M van Leeuwen
Feb 22, 2017·Journal of Clinical Laboratory Analysis·Yu-Juan ChenShu-Hua Zhang
Apr 17, 2013·Journal of Cellular Physiology·V J WoeckelJ P T M Van Leeuwen
Jan 27, 1998·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·A StaalJ P Van Leeuwen
Aug 10, 2007·Nephron. Physiology·Masaki NakaneRuth Wu-Wong
Mar 3, 2010·European Journal of Clinical Investigation·Panagiota GeorgiadouDimitrios T Kremastinos
Apr 6, 2019·The Journals of Gerontology. Series A, Biological Sciences and Medical Sciences·Monica CastleSue A Shapses
Mar 8, 2014·BoneKEy Reports·Marjolein van Driel, Johannes P T M van Leeuwen
May 20, 2005·Endocrine Reviews·Katrien JanssensWim Van Hul
Mar 24, 1999·Journal of Cellular Biochemistry·C FaucheuxJ T Triffitt
Aug 15, 2003·Immunological Investigations·Cheorl-Ho KimYoung-Guk Park
Nov 25, 2003·The Journal of Biological Chemistry·Sergio Roman-RomanGeorges Rawadi

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