Antagonistic roles for the ubiquitin ligase Asr1 and the ubiquitin-specific protease Ubp3 in subtelomeric gene silencing

Proceedings of the National Academy of Sciences of the United States of America
Tyler S McCannWilliam P Tansey

Abstract

Ubiquitin, and components of the ubiquitin-proteasome system, feature extensively in the regulation of gene transcription. Although there are many examples of how ubiquitin controls the activity of transcriptional regulators and coregulators, there are few examples of core components of the transcriptional machinery that are directly controlled by ubiquitin-dependent processes. The budding yeast protein Asr1 is the prototypical member of the RPC (RING, PHD, CBD) family of ubiquitin-ligases, characterized by the presence of amino-terminal RING (really interesting new gene) and PHD (plant homeo domain) fingers and a carboxyl-terminal domain that directly binds the largest subunit of RNA polymerase II (pol II), Rpb1, in response to phosphorylation events tied to the initiation of transcription. Asr1-mediated oligo-ubiquitylation of pol II leads to ejection of two core subunits of the enzyme and is associated with inhibition of polymerase function. Here, we present evidence that Asr1-mediated ubiquitylation of pol II is required for silencing of subtelomeric gene transcription. We show that Asr1 associates with telomere-proximal chromatin and that disruption of the ubiquitin-ligase activity of Asr1--or mutation of ubiquitylation si...Continue Reading

References

Dec 16, 1997·Proceedings of the National Academy of Sciences of the United States of America·E K MonsonV A Zakian
Jun 13, 2002·Proceedings of the National Academy of Sciences of the United States of America·Michael J MacCossJohn R Yates
Jan 1, 2003·Journal of Cell Science·Jesper Q Svejstrup
Feb 18, 2004·Molecular Cell·Elena Ezhkova, William P Tansey
Jan 27, 2006·Molecular Biology of the Cell·Astrid S ClarkeLorraine Pillus
Oct 24, 2006·Proceedings of the National Academy of Sciences of the United States of America·Carole GwizdekCatherine Dargemont
Jan 16, 2008·Molecular and Cellular Biology·Vanessa M RunnerStephen Buratowski
May 24, 2008·Molecular Cell·Kristian KvintJesper Q Svejstrup
Dec 10, 2008·Proceedings of the National Academy of Sciences of the United States of America·Anne DaulnyWilliam P Tansey
Mar 13, 2012·Annual Review of Biochemistry·Fuqiang GengWilliam P Tansey
Sep 27, 2013·Briefings in Bioinformatics·Yan GuoDavid C Samuels

❮ Previous
Next ❯

Citations

Nov 24, 2020·ELife·Fanny EybouletFrançois Robert

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Proceedings of the National Academy of Sciences of the United States of America
Anne DaulnyWilliam P Tansey
Proceedings of the National Academy of Sciences of the United States of America
J M HuibregtseS L Beaudenon
© 2022 Meta ULC. All rights reserved