Antagonistic roles of SEPALLATA3, FT and FLC genes as targets of the polycomb group gene CURLY LEAF.

PloS One
Manuel Lopez-VernazaJustin Goodrich

Abstract

In Arabidopsis, mutations in the Pc-G gene CURLY LEAF (CLF) give early flowering plants with curled leaves. This phenotype is caused by mis-expression of the floral homeotic gene AGAMOUS (AG) in leaves, so that ag mutations largely suppress the clf phenotype. Here, we identify three mutations that suppress clf despite maintaining high AG expression. We show that the suppressors correspond to mutations in FPA and FT, two genes promoting flowering, and in SEPALLATA3 (SEP3) which encodes a co-factor for AG protein. The suppression of the clf phenotype is correlated with low SEP3 expression in all case and reveals that SEP3 has a role in promoting flowering in addition to its role in controlling floral organ identity. Genetic analysis of clf ft mutants indicates that CLF promotes flowering by reducing expression of FLC, a repressor of flowering. We conclude that SEP3 is the key target mediating the clf phenotype, and that the antagonistic effects of CLF target genes masks a role for CLF in promoting flowering.

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Citations

Oct 7, 2015·Photochemistry and Photobiology·Christian D LorenzoPablo D Cerdán
May 28, 2013·Journal of Genetics and Genomics = Yi Chuan Xue Bao·Anne Molitor, Wen-Hui Shen
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Apr 22, 2017·Proceedings of the National Academy of Sciences of the United States of America·Yue ZhouFranziska Turck
Sep 29, 2019·Journal of Experimental Botany·Kai ChengMing Luo
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Mar 1, 2021·Current Opinion in Plant Biology·Margaret Anne PelayoToshiro Ito
Sep 11, 2021·The Plant Journal : for Cell and Molecular Biology·Suthitar Singkaravanit-OgawaYoshitaka Takano

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Methods Mentioned

BETA
transgenic
PCR
immunoprecipitation
ChIP

Software Mentioned

Lightcycler 480

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