Antarctic krill population genomics: apparent panmixia, but genome complexity and large population size muddy the water

Molecular Ecology
Bruce E DeagleS Jarman

Abstract

Antarctic krill (Euphausia superba; hereafter krill) are an incredibly abundant pelagic crustacean which has a wide, but patchy, distribution in the Southern Ocean. Several studies have examined the potential for population genetic structuring in krill, but DNA-based analyses have focused on a limited number of markers and have covered only part of their circum-Antarctic range. We used mitochondrial DNA and restriction site-associated DNA sequencing (RAD-seq) to investigate genetic differences between krill from five sites, including two from East Antarctica. Our mtDNA results show no discernible genetic structuring between sites separated by thousands of kilometres, which is consistent with previous studies. Using standard RAD-seq methodology, we obtained over a billion sequences from >140 krill, and thousands of variable nucleotides were identified at hundreds of loci. However, downstream analysis found that markers with sufficient coverage were primarily from multicopy genomic regions. Careful examination of these data highlights the complexity of the RAD-seq approach in organisms with very large genomes. To characterize the multicopy markers, we recorded sequence counts from variable nucleotide sites rather than the derived...Continue Reading

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Citations

Jan 13, 2017·Molecular Ecology Resources·Alison G NazarenoLúcia G Lohmann
Dec 23, 2020·Molecular Ecology Resources·Roberta GargiuloMichael F Fay
Dec 11, 2020·Molecular Ecology·Laurence J ClarkeBruce E Deagle
Oct 23, 2021·The Journal of Animal Ecology·Kara K S Layton, Ian R Bradbury

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