Antarease-like Zn-metalloproteases are ubiquitous in the venom of different scorpion genera

Biochimica Et Biophysica Acta
Ernesto OrtizL D Possani

Abstract

The venoms of several scorpion species have long been associated with pancreatitis in animal models and humans. Antarease, a Zn-metalloprotease from Tityus serrulatus, is able to penetrate intact pancreatic tissue and disrupts the normal vesicular traffic necessary for secretion, so it could play a relevant role in the onset of acute pancreatitis. The cDNA libraries from five different scorpion species were screened for antarease homologs with specific primers. The amplified PCR products were cloned and sequenced. A structural model was constructed to assess the functionality of the putative metalloproteases. A phylogenetic analysis was performed to identify clustering patterns of these venom components. Antarease-like sequences were amplified from all the screened cDNA libraries. The complete sequence of the antarease from T. serrulatus was obtained. The structural model of the putative antarease from Tityus trivittatus shows that it may adopt a catalytically active conformation, sharing relevant structural elements with previously reported metalloproteases of the ADAM family. The phylogenetic analysis reveals that the reported sequences cluster in groups that correlate with the geographical localization of the respective spec...Continue Reading

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Citations

Dec 30, 2015·Toxins·Carlos E Santibáñez-LópezLourival D Possani
Feb 28, 2016·Toxicon : Official Journal of the International Society on Toxinology·Timoteo Olamendi-PortugalLourival D Possani
Aug 6, 2014·Toxicon : Official Journal of the International Society on Toxinology·A O CarmoE Kalapothakis
Feb 7, 2015·PloS One·Verónica Quintero-HernándezErnesto Ortiz
May 29, 2015·PloS One·Karen Luna-RamírezLourival D Possani
Sep 23, 2014·Toxicological Sciences : an Official Journal of the Society of Toxicology·Daniela Cajado CarvalhoFernanda V Portaro
Mar 22, 2018·PloS One·Ursula Castro de OliveiraInácio de Loiola Meirelles Junqueira-de-Azevedo
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Dec 14, 2019·Expert Review of Proteomics·Jimena I Cid-UribeLourival D Possani
May 5, 2016·Anais Da Academia Brasileira De Ciências·Nathalia A SilvaRené D Martins
Aug 6, 2020·Biomedicines·Edward R J EvansDavid T Wilson
May 28, 2019·The Journal of Venomous Animals and Toxins Including Tropical Diseases·Fernanda Gobbi AmorimEliane Candiani Arantes
Mar 4, 2020·Toxicon : Official Journal of the International Society on Toxinology·Laura L Valdez-VelázquezLourival D Possani
Jun 26, 2020·Toxicon : Official Journal of the International Society on Toxinology·Allanny Alves FurtadoMatheus de Freitas Fernandes-Pedrosa

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