Ante-mortem detection of chronic wasting disease in recto-anal mucosa-associated lymphoid tissues from elk (Cervus elaphus nelsoni) using real-time quaking-induced conversion (RT-QuIC) assay: A blinded collaborative study

Prion
Sireesha ManneAnumantha G Kanthasamy

Abstract

Prion diseases are transmissible spongiform encephalopathies (TSEs) characterized by fatal, progressive neurologic diseases with prolonged incubation periods and an accumulation of infectious misfolded prion proteins. Antemortem diagnosis is often difficult due to a long asymptomatic incubation period, differences in the pathogenesis of different prions, and the presence of very low levels of infectious prion in easily accessible samples. Chronic wasting disease (CWD) is a TSE affecting both wild and captive populations of cervids, including mule deer, white-tailed deer, elk, moose, muntjac, and most recently, wild reindeer. This study represents a well-controlled evaluation of a newly developed real-time quaking-induced conversion (RT-QuIC) assay as a potential CWD diagnostic screening test using rectal biopsy sections from a depopulated elk herd. We evaluated 69 blinded samples of recto-anal mucosa-associated lymphoid tissue (RAMALT) obtained from USDA Veterinary Services. The results were later un-blinded and statistically compared to immunohistochemical (IHC) results from the USDA National Veterinary Services Laboratories (NVSL) for RAMALT, obex, and medial retropharyngeal lymph node (MRPLN). Comparison of RAMALT RT-QuIC as...Continue Reading

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Citations

Nov 24, 2019·Movement Disorders : Official Journal of the Movement Disorder Society·Sireesha ManneAnumantha G Kanthasamy
Sep 23, 2020·Movement Disorders : Official Journal of the Movement Disorder Society·Sireesha ManneAnumantha G Kanthasamy
Feb 2, 2019·Journal of Neuroimmune Pharmacology : the Official Journal of the Society on NeuroImmune Pharmacology·Sireesha ManneAnumantha G Kanthasamy
Nov 27, 2020·Frontiers in Veterinary Science·Najiba MammadovaJustin J Greenlee
Jan 28, 2022·Cell and Tissue Research·Ryuichiro Atarashi

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Methods Mentioned

BETA
biopsies
ELISA
NMR
genotyping
Dissection
biopsy
Fluorescence
Nuclear
Circular Dichroism
electrophoresis

Software Mentioned

RT
Prism
obex
RAMALT RT - QuIC
QuIC
GraphPad
RAMALT
RAMALT - QuIC
MRPLN

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