Antenna effect and phosphorescence spectra to find the location of drug tetracycline in bovine β-lactoglobulin A

Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry
Moumita MukherjeeSanjib Ghosh

Abstract

A ternary system comprising of a Eu(III) complex of the drug Tetracycline hydrochloride (Eu3TC) bound to bovine β-lactoglobulin variant A (BLGA) in aqueous buffer at physiological pH (pH = 7.4) has been investigated to exploit the enhanced "antenna effect" to locate the bound drug and find the microenvironment of the binding site. Steady-state and time-resolved emission studies at room temperature as well as at 77 K have been carried out to evaluate the binding parameters in the binary system consisting of BLGA and tetracycline hydrochloride (TC). Low-temperature phosphorescence studies at 77 K of pure BLGA confirm Trp 19 to be the emitting residue, while Trp 61 is silent. Enhancement of BLGA phosphorescence emission in the ternary system at 77 K indicates that Trp 19 is very close to Eu(III) in the Eu3TC complex. The molecular docking results further confirm that TC binds close to Trp 19 in a hydrophobic domain. The results thus obtained can provide guidelines to design and synthesize target-oriented drugs as well as suitable bio-probes.

References

Aug 23, 1996·Journal of Molecular Biology·M RareyG Klebe
Dec 1, 1996·Critical Reviews in Food Science and Nutrition·D W WongA E Pavlath
Dec 29, 1998·The Journal of Biological Chemistry·S Y WuL Sawyer
Dec 11, 1999·Nucleic Acids Research·H M BermanP E Bourne
Nov 4, 2000·Biochimica Et Biophysica Acta·L Sawyer, G Kontopidis
Jul 31, 2001·The Journal of Laboratory and Clinical Medicine·J B RubinsR A Kratzke
Jun 11, 2002·Journal of Molecular Biology·George KontopidisLindsay Sawyer
Mar 26, 2004·Biophysical Journal·Maria Isabel ViseuSílvia M B Costa
Jul 21, 2004·Journal of Computational Chemistry·Eric F PettersenThomas E Ferrin
Jan 15, 2005·Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy·Shuyun BiHanqi Zhang
Feb 16, 2007·The Journal of Physical Chemistry. B·Billie J HarveyLorenzo Brancaleon
Mar 25, 2008·Biochimica Et Biophysica Acta·P A T MartinsM J Moreno
Jul 21, 2012·The Journal of Physical Chemistry. a·Shyamal Kr GhoraiSanjib Ghosh
Jan 28, 2014·Journal of Photochemistry and Photobiology. B, Biology·Shyamal Kr GhoraiSanjib Ghosh
Jan 24, 2015·Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy·F MohammadiA Khalegh Bordbar
May 7, 2016·Nucleic Acids Research·Douglas E V Pires, David B Ascher
Aug 27, 2016·Journal of Biomolecular Structure & Dynamics·Mohammad Hossein MehrabanYounes Ghasemi

❮ Previous
Next ❯

Citations

Aug 9, 2021·Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy·Moumita MukherjeeSanjib Ghosh

❮ Previous
Next ❯

Methods Mentioned

BETA
X-ray

Software Mentioned

UCSF Chimera
mathop
Sybyl
CSM
PicoQuant
FlexX
NACCESS
lig
Tripos

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.