PMID: 9182848Jun 30, 1997Paper

Antennal lobe neurons of the honey bee, Apis mellifera, express a D2-like dopamine receptor in vitro

The Journal of Comparative Neurology
B S Kirchhof, Alison Mercer

Abstract

We have used the D2-specific dopamine receptor ligand spiperone [N-(p-aminophenethyl) spiperone; NAPS] coupled to the fluorophore 7-nitrobenz-2-oxa-1,3-diazole-4-yl (NBD) to visualize dopamine receptors expressed in vitro by neurons of the primary antennosensory centers (antennal lobes) of the brain of the honey bee, Apis mellifera. Changes in the percentage of antennal lobe neurons exhibiting spiperone binding sites over time in culture and at different stages of metamorphic adult development have been investigated. Neurons obtained from animals at all stages of development exhibited spiperone binding sites, but only after 2 days or more in vitro. The percentage of antennal lobe neurons in vitro expressing spiperone binding sites increased significantly with the development of the antennal lobe neuropil. Fluorescently labelled spiperone (120 nM) could be displaced effectively by 1 mM dopamine but not by the same concentration of tyramine, octopamine, or serotonin. In addition, the D2 antagonist spiperone and the D2/D1 antagonist fluphenazine were more effective at displacing the fluorescent ligand than the D1-specific antagonist SCH23390. Our results indicate that Apis antennal lobe neurons in culture express a dopamine recept...Continue Reading

References

Aug 1, 1992·Journal of Neurocytology·S Kreissl, G Bicker
Jul 1, 1992·Journal of Comparative Physiology. A, Sensory, Neural, and Behavioral Physiology·D J TaylorA R Mercer
Dec 20, 1990·The Journal of Steroid Biochemistry and Molecular Biology·R C CoombesY Luqmani
Jan 1, 1990·Physiological Reviews·H Mustaparta
Nov 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·M A ArianoD R Sibley
Jan 1, 1989·Annual Review of Entomology·U HombergJ G Hildebrand
Feb 1, 1989·The Journal of Comparative Neurology·S Schäfer, V Rehder
Feb 15, 1988·The Journal of Comparative Neurology·V Budnik, K White
Jan 1, 1984·Progress in Neurobiology·J J Feigenbaum, J Yanai
Dec 1, 1994·The Journal of Comparative Neurology·L MedinaW J Smeets
Feb 15, 1993·Microscopy Research and Technique·J Boeckh, L P Tolbert
Jan 1, 1993·Annual Review of Neuroscience·J A Gingrich, M G Caron
May 8, 1993·The Journal of Comparative Neurology·Z Muresan, J C Besharse
Jan 1, 1996·Journal of Comparative Physiology. A, Sensory, Neural, and Behavioral Physiology·A R MercerJ G Hildebrand
Jan 8, 1996·Brain Research·I C Kokay, A R Mercer
Jan 1, 1996·Behavioural Brain Research·P M Whitaker-AzmitiaJ M Lauder

❮ Previous
Next ❯

Citations

Jun 10, 2005·Insect Biochemistry and Molecular Biology·Kyle T BeggsAlison R Mercer
Jul 27, 2005·Journal of Comparative Physiology. A, Neuroethology, Sensory, Neural, and Behavioral Physiology·Guillaume Stephane BarbaraBernd Grünewald
Nov 12, 2005·Journal of Neurophysiology·Christopher G Perk, Alison R Mercer
Aug 8, 2013·Archives of Insect Biochemistry and Physiology·Daniel E WilsonGuy Smagghe
May 13, 1998·Behavioural Brain Research·S M MorganA R Mercer

❮ Previous
Next ❯

Related Concepts

Related Feeds

Antipsychotic Drugs

Antipsychotic drugs are a class of medication primarily used to manage psychosis (including delusions, hallucinations, paranoia or disordered thought), principally in schizophrenia and bipolar disorder. Discover the latest research on antipsychotic drugs here