Anterograde monosynaptic transneuronal tracers derived from herpes simplex virus 1 strain H129

Molecular Neurodegeneration
Wen-Bo ZengMin-Hua Luo

Abstract

Herpes simplex virus type 1 strain 129 (H129) has represented a promising anterograde neuronal circuit tracing tool, which complements the existing retrograde tracers. However, the current H129 derived tracers are multisynaptic, neither bright enough to label the details of neurons nor capable of determining direct projection targets as monosynaptic tracer. Based on the bacterial artificial chromosome of H129, we have generated a serial of recombinant viruses for neuronal circuit tracing. Among them, H129-G4 was obtained by inserting binary tandemly connected GFP cassettes into the H129 genome, and H129-ΔTK-tdT was obtained by deleting the thymidine kinase (TK) gene and adding tdTomato coding gene to the H129 genome. Then the obtained viral tracers were tested in vitro and in vivo for the tracing capacity. H129-G4 is capable of transmitting through multiple synapses, labeling the neurons by green florescent protein, and visualizing the morphological details of the labeled neurons. H129-ΔTK-tdT neither replicates nor spreads in neurons alone, but transmits to and labels the postsynaptic neurons with tdTomato in the presence of complementary expressed TK from a helper virus. H129-ΔTK-tdT is also capable to map the direct projecto...Continue Reading

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Citations

Mar 1, 2019·The Journal of Immunology : Official Journal of the American Association of Immunologists·Meng-Xin ZhangDandan Lin
Jun 12, 2018·Frontiers in Neural Circuits·Suellen Almeida-CorrêaCarsten T Wotjak
May 30, 2020·Virologica Sinica·Wen-Bo ZengMin-Hua Luo
Sep 12, 2019·Frontiers in Neuroscience·Christine SaleebaSimon McMullan
Feb 10, 2019·Molecular Neurodegeneration·Leqiang SunGang Cao
Dec 21, 2020·Journal of Neuroscience Research·Chase A Weinholtz, Michael J Castle
Mar 24, 2021·Journal of Neuroscience Methods·Oscar M T ChadneyClifford G Kentros

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Methods Mentioned

BETA
transgenic
dissection
PCR

Software Mentioned

fMOST
Amira

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